M00149-1

antibody from Boster Biological Technology

Targeting: CCND1 BCL1, D11S287E, PRAD1, U21B31

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00149-1

Provider

Boster Biological Technology

Product name

Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Cyclin D1/CCND1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat Cyclin D1/CCND1 information: Molecular Weight: 33729 MW; Subcellular Localization: Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DAD-3

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody(M00149-1)CCND1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CCND1 Antibody (M00149-1)overnight at 4

Additional image