LS-B16338

antibody from LSBio

Targeting: CCND1 BCL1, D11S287E, PRAD1, U21B31

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B16338

Provider

LSBio

Product name

IHC-plus™ CCND1 / Cyclin D1 Antibody (clone 3B6G3) LS-B16338

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3B6G3

Storage

Short term: store at 4°C. Long term: store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using CCND1 mAb against HEK293 (1) and CCND1 (AA: 167-295)-hIgGFc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using CCND1 mAb against human CCND1 (AA: 167-295) recombinant protein. (Expected MW is 40.1 kDa)

ELISA

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CCND1 mouse mAb with DAB staining.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence analysis of Hela cells using CCND1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CCND1 mouse mAb with DAB staining.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CCND1 mouse mAb with DAB staining.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence analysis of Hela cells using CCND1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher