ADI-KAM-CC200-E

antibody from Enzo Life Sciences

Targeting: CCND1 BCL1, D11S287E, PRAD1, U21B31

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ADI-KAM-CC200-E

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-KAM-CC200-E, RRID:AB_2039040

Product name

Cyclin D1 monoclonal antibody (5D4)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Description

Protein A affinity purified.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

5D4

Vial size

100 μg

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of ZR- 75-1 (1), MCF7 (2), HeLa (3), WR19L (4) and NIH/3T3 (5) cells probed with Cyclin D1 mAb (5D4).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cyclin D1 on paraffin embedded section of human mantle cell lymphoma with Cyclin D1 mAb (5D4).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunoprecipitation analysis of cyclin D1 from ZR75-1 cells with Mouse IgG2a (1) or Cyclin D1 mAb (5D4) (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoBlotted with 553.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Flow cytometry analysis of Cyclin D1 expression in HeLa cells. Open histogram indicates the reaction of Isotypic control to the cells. Shaded histogram indicates the reaction of Cyclin D1 mAb (5D4) to the cells.