AP1253E

antibody from Abgent

Targeting: MDM2 HDM2, MGC5370

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1253E

Provider

Abgent

Proper citation

Abgent Cat#AP1253e, RRID:AB_1552978

Product name

MDM2 Antibody (S166)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

RB7981

Vial size

400 µl

Storage

Store at 2 to 8°C.Antibody is stable for 50 months.

References

Submitted references

Pathological signaling via platelet-derived growth factor receptor {alpha} involves chronic activation of Akt and suppression of p53.

Lei H, Velez G, Kazlauskas A
Molecular and cellular biology 2011 May;31(9):1788-99

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of MDM2 antibody (S166) (Cat. #AP1253e) in 293 cell line lysates (35ug/lane). MDM2 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of MDM2 Antibody (S166) (Cat. #AP1253e) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the MDM2 Antibody (S166) (Cat.#AP1253e), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50