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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP1254a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1254a, RRID:AB_2143554
- Product name
- Mdm2 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- RB00757
- Vial size
- 400 µl
- Storage
- Store at 2 to 8°C.Antibody is stable for 51 months.
Submitted references The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2.
Stevenson LF, Sparks A, Allende-Vega N, Xirodimas DP, Lane DP, Saville MK
The EMBO journal 2007 Feb 21;26(4):976-86
The EMBO journal 2007 Feb 21;26(4):976-86
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of MDM2 (arrow) using rabbit polyclonal Mdm2 Antibody (C-term) (Cat.#AP1254a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDM2 gene (Lane 2) (Origene Technologies).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-Mdm2 Pab (Cat. #AP1254a) in mouse lung tissue lysate. Mdm2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- MDM2 Antibody (Q213) (Cat.#AP1254a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MDM2 antibody detected the MDM2 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Mdm2 Antibody (C-term) (Cat.#AP1254a) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Mdm2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
