A00054-1

antibody from Boster Biological Technology

Targeting: MDM2 HDM2, MGC5370

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00054-1

Provider

Boster Biological Technology

Product name

Anti-MDM2 (Ab-166) Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for Q00987 detection. Tested positive for WB IHC IF in Hu

Reactivity

Human

Host

Rabbit

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store at -20°C for long term preservation (recommended) . Store at 4°C for short term use.

References

Submitted references

MDM2 promotes epithelial-mesenchymal transition through activation of Smad2/3 signaling pathway in lung adenocarcinoma.

Tang Y, Xuan Y, Qiao G, Ou Z, He Z, Zhu Q, Liao M, Yin G
OncoTargets and therapy 2019;12:2247-2258

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of MDM2 using anti-MDM2 antibody (A00054-1).MDM2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MDM2 Antibody (A00054-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image