Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-2736 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2736, RRID:AB_10001211
- Product name
- Mouse Monoclonal MDM2/HDM2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Antigen sequence
Amino acids 154 - 167.- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Expression of MDM2 in Macrophages Promotes the Early Postentry Steps of HIV-1 Infection through Inhibition of p53.
Thyroid hormone upregulates MDM2 in rat type I fibre: Implications for skeletal muscle mass regulation.
The MDM4/MDM2-p53-IGF1 axis controls axonal regeneration, sprouting and functional recovery after CNS injury.
Identification of signaling pathways mediating cell cycle arrest and apoptosis induced by Porphyromonas gingivalis in human trophoblasts.
p53 and MDM2 protein expression in actinic cheilitis.
p53 and MDM2 protein expression in actinic cheilitis.
The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2.
Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
HMG-CoA reductase inhibitors, statins, induce phosphorylation of Mdm2 and attenuate the p53 response to DNA damage.
The RING domain of Mdm2 mediates histone ubiquitylation and transcriptional repression.
Nuclear export inhibitor leptomycin B induces the appearance of novel forms of human Mdm2 protein.
Role of P53 and MDM2 in treatment response of human germ cell tumors.
Tyrosine phosphorylation of Mdm2 by c-Abl: implications for p53 regulation.
MDM2 mediated nuclear exclusion of p53 attenuates etoposide-induced apoptosis in neuroblastoma cells.
MDM2 mediated nuclear exclusion of p53 attenuates etoposide-induced apoptosis in neuroblastoma cells.
A role for the polyproline domain of p53 in its regulation by Mdm2.
Phosphorylation of murine double minute clone 2 (MDM2) protein at serine-267 by protein kinase CK2 in vitro and in cultured cells.
Phosphorylation of murine double minute clone 2 (MDM2) protein at serine-267 by protein kinase CK2 in vitro and in cultured cells.
c-Abl neutralizes the inhibitory effect of Mdm2 on p53.
c-Abl neutralizes the inhibitory effect of Mdm2 on p53.
DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2.
DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2.
Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein.
Breton Y, Desrosiers V, Ouellet M, Deshiere A, Torresilla C, Cohen ÉA, Tremblay MJ
Journal of virology 2019 Apr 1;93(7)
Journal of virology 2019 Apr 1;93(7)
Thyroid hormone upregulates MDM2 in rat type I fibre: Implications for skeletal muscle mass regulation.
Ramos GV, Cruz A, Silva WJ, Rozanski A, Baptista IL, Silvestre JG, Moriscot AS
Acta physiologica (Oxford, England) 2018 Apr;222(4):e13003
Acta physiologica (Oxford, England) 2018 Apr;222(4):e13003
The MDM4/MDM2-p53-IGF1 axis controls axonal regeneration, sprouting and functional recovery after CNS injury.
Joshi Y, Sória MG, Quadrato G, Inak G, Zhou L, Hervera A, Rathore KI, Elnaggar M, Cucchiarini M, Marine JC, Puttagunta R, Di Giovanni S
Brain : a journal of neurology 2015 Jul;138(Pt 7):1843-62
Brain : a journal of neurology 2015 Jul;138(Pt 7):1843-62
Identification of signaling pathways mediating cell cycle arrest and apoptosis induced by Porphyromonas gingivalis in human trophoblasts.
Inaba H, Kuboniwa M, Sugita H, Lamont RJ, Amano A
Infection and immunity 2012 Aug;80(8):2847-57
Infection and immunity 2012 Aug;80(8):2847-57
p53 and MDM2 protein expression in actinic cheilitis.
de Freitas Mda C, Ramalho LM, Xavier FC, Moreira AL, Reis SR
Journal of applied oral science : revista FOB 2008 Nov-Dec;16(6):414-9
Journal of applied oral science : revista FOB 2008 Nov-Dec;16(6):414-9
p53 and MDM2 protein expression in actinic cheilitis.
de Freitas Mda C, Ramalho LM, Xavier FC, Moreira AL, Reis SR
Journal of applied oral science : revista FOB 2008 Nov-Dec;16(6):414-9
Journal of applied oral science : revista FOB 2008 Nov-Dec;16(6):414-9
The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2.
Stevenson LF, Sparks A, Allende-Vega N, Xirodimas DP, Lane DP, Saville MK
The EMBO journal 2007 Feb 21;26(4):976-86
The EMBO journal 2007 Feb 21;26(4):976-86
Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function.
Chen D, Zhang Z, Li M, Wang W, Li Y, Rayburn ER, Hill DL, Wang H, Zhang R
Oncogene 2007 Aug 2;26(35):5029-37
Oncogene 2007 Aug 2;26(35):5029-37
HMG-CoA reductase inhibitors, statins, induce phosphorylation of Mdm2 and attenuate the p53 response to DNA damage.
Pääjärvi G, Roudier E, Crisby M, Högberg J, Stenius U
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2005 Mar;19(3):476-8
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2005 Mar;19(3):476-8
The RING domain of Mdm2 mediates histone ubiquitylation and transcriptional repression.
Minsky N, Oren M
Molecular cell 2004 Nov 19;16(4):631-9
Molecular cell 2004 Nov 19;16(4):631-9
Nuclear export inhibitor leptomycin B induces the appearance of novel forms of human Mdm2 protein.
Menéndez S, Higgins M, Berkson RG, Edling C, Lane DP, Laín S
British journal of cancer 2003 Feb 24;88(4):636-43
British journal of cancer 2003 Feb 24;88(4):636-43
Role of P53 and MDM2 in treatment response of human germ cell tumors.
Kersemaekers AM, Mayer F, Molier M, van Weeren PC, Oosterhuis JW, Bokemeyer C, Looijenga LH
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2002 Mar 15;20(6):1551-61
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2002 Mar 15;20(6):1551-61
Tyrosine phosphorylation of Mdm2 by c-Abl: implications for p53 regulation.
Goldberg Z, Vogt Sionov R, Berger M, Zwang Y, Perets R, Van Etten RA, Oren M, Taya Y, Haupt Y
The EMBO journal 2002 Jul 15;21(14):3715-27
The EMBO journal 2002 Jul 15;21(14):3715-27
MDM2 mediated nuclear exclusion of p53 attenuates etoposide-induced apoptosis in neuroblastoma cells.
Rodriguez-Lopez AM, Xenaki D, Eden TO, Hickman JA, Chresta CM
Molecular pharmacology 2001 Jan;59(1):135-43
Molecular pharmacology 2001 Jan;59(1):135-43
MDM2 mediated nuclear exclusion of p53 attenuates etoposide-induced apoptosis in neuroblastoma cells.
Rodriguez-Lopez AM, Xenaki D, Eden TO, Hickman JA, Chresta CM
Molecular pharmacology 2001 Jan;59(1):135-43
Molecular pharmacology 2001 Jan;59(1):135-43
A role for the polyproline domain of p53 in its regulation by Mdm2.
Berger M, Vogt Sionov R, Levine AJ, Haupt Y
The Journal of biological chemistry 2001 Feb 9;276(6):3785-90
The Journal of biological chemistry 2001 Feb 9;276(6):3785-90
Phosphorylation of murine double minute clone 2 (MDM2) protein at serine-267 by protein kinase CK2 in vitro and in cultured cells.
Hjerrild M, Milne D, Dumaz N, Hay T, Issinger OG, Meek D
The Biochemical journal 2001 Apr 15;355(Pt 2):347-56
The Biochemical journal 2001 Apr 15;355(Pt 2):347-56
Phosphorylation of murine double minute clone 2 (MDM2) protein at serine-267 by protein kinase CK2 in vitro and in cultured cells.
Hjerrild M, Milne D, Dumaz N, Hay T, Issinger OG, Meek D
The Biochemical journal 2001 Apr 15;355(Pt 2):347-56
The Biochemical journal 2001 Apr 15;355(Pt 2):347-56
c-Abl neutralizes the inhibitory effect of Mdm2 on p53.
Sionov RV, Moallem E, Berger M, Kazaz A, Gerlitz O, Ben-Neriah Y, Oren M, Haupt Y
The Journal of biological chemistry 1999 Mar 26;274(13):8371-4
The Journal of biological chemistry 1999 Mar 26;274(13):8371-4
c-Abl neutralizes the inhibitory effect of Mdm2 on p53.
Sionov RV, Moallem E, Berger M, Kazaz A, Gerlitz O, Ben-Neriah Y, Oren M, Haupt Y
The Journal of biological chemistry 1999 Mar 26;274(13):8371-4
The Journal of biological chemistry 1999 Mar 26;274(13):8371-4
DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2.
Shieh SY, Ikeda M, Taya Y, Prives C
Cell 1997 Oct 31;91(3):325-34
Cell 1997 Oct 31;91(3):325-34
DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2.
Shieh SY, Ikeda M, Taya Y, Prives C
Cell 1997 Oct 31;91(3):325-34
Cell 1997 Oct 31;91(3):325-34
Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein.
Martin K, Trouche D, Hagemeier C, Sørensen TS, La Thangue NB, Kouzarides T
Nature 1995 Jun 22;375(6533):691-4
Nature 1995 Jun 22;375(6533):691-4
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Daudi whole cell protein was separated by SDS-PAGE on a 7.5% gel and transferred to PVDF membrane. The membrane was probed with anti-MDM2 antibody at 2 ug/ml and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody (SMP14) [NB100-2736] - Analysis of tissue section of human breast cancer xenograft using MDM2/HDM2 antibody (clone SMP14) at 1:100 dilution. Several of the cancer cells developed a strong nuclear with weak cytoplasmic immunostaining of MDM2.
