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LearnNBP1-33003
antibody from Novus Biologicals
Targeting: TAGLN
DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [5]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-33003 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33003, RRID:AB_2199494
- Product name
- Rabbit Polyclonal Transgelin/TAGLN/SM22 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references H19/TET1 axis promotes TGF-β signaling linked to endothelial-to-mesenchymal transition.
βklotho is essential for the anti-endothelial mesenchymal transition effects of N-acetyl-seryl-aspartyl-lysyl-proline.
FGFR1 is critical for the anti-endothelial mesenchymal transition effect of N-acetyl-seryl-aspartyl-lysyl-proline via induction of the MAP4K4 pathway.
Interactions of DPP-4 and integrin β1 influences endothelial-to-mesenchymal transition.
Cao T, Jiang Y, Li D, Sun X, Zhang Y, Qin L, Tellides G, Taylor HS, Huang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jun;34(6):8625-8640
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jun;34(6):8625-8640
βklotho is essential for the anti-endothelial mesenchymal transition effects of N-acetyl-seryl-aspartyl-lysyl-proline.
Gao R, Kanasaki K, Li J, Kitada M, Okazaki T, Koya D
FEBS open bio 2019 May;9(5):1029-1038
FEBS open bio 2019 May;9(5):1029-1038
FGFR1 is critical for the anti-endothelial mesenchymal transition effect of N-acetyl-seryl-aspartyl-lysyl-proline via induction of the MAP4K4 pathway.
Li J, Shi S, Srivastava SP, Kitada M, Nagai T, Nitta K, Kohno M, Kanasaki K, Koya D
Cell death & disease 2017 Aug 3;8(8):e2965
Cell death & disease 2017 Aug 3;8(8):e2965
Interactions of DPP-4 and integrin β1 influences endothelial-to-mesenchymal transition.
Shi S, Srivastava SP, Kanasaki M, He J, Kitada M, Nagai T, Nitta K, Takagi S, Kanasaki K, Koya D
Kidney international 2015 Sep;88(3):479-89
Kidney international 2015 Sep;88(3):479-89
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - 30 ug C8D30 whole cell lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (15 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - A. 30 ug Rat-2 whole cell lysate/extract 12 % SDS-PAGE Transgelin antibody dilution: 1:5000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Immunoprecipitation of Transgelin protein from HeLa whole cell extracts using 5 ug of Transgelin antibody \ Western blot analysis was performed using Transgelin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Rat stomach. Transgelin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
