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LearnPA5-27463
antibody from Invitrogen Antibodies
Targeting: TAGLN
DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-27463 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAGLN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, C8D30, Rat-2.
- Concentration
- 1.22 mg/mL
Submitted references A protocol for rapid pericyte differentiation of human induced pluripotent stem cells.
Aisenbrey EA, Torr E, Johnson H, Soref C, Daly W, Murphy WL
STAR protocols 2021 Mar 19;2(1):100261
STAR protocols 2021 Mar 19;2(1):100261
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SM22 alpha using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SM22 alpha polyclonal antibody (Product # PA5-27463) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TAGLN Polyclonal Antibody (Product # PA5-27463). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TAGLN Polyclonal Antibody (Product # PA5-27463) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TAGLN Polyclonal Antibody detects TAGLN protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract.12% SDS-PAGE. TAGLN Polyclonal Antibody (Product # PA5-27463) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TAGLN Polyclonal Antibody detects TAGLN protein by western blot analysis. A. 30 µg Rat-2 whole cell lysate/extract.12% SDS-PAGE. TAGLN Polyclonal Antibody (Product # PA5-27463) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TAGLN was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TAGLN Polyclonal Antibody (Product # PA5-27463) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TAGLN was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TAGLN Polyclonal Antibody (Product # PA5-27463) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TAGLN was performed in paraffin-embedded rat stomach tissue using TAGLN Polyclonal Antibody (Product # PA5-27463) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TAGLN Polyclonal Antibody detects Transgelin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse smooth muscle. Transgelin stained by TAGLN Polyclonal Antibody (Product # PA5-27463) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Transgelin was performed in HeLa whole cell extracts using 5 µg of TAGLN Polyclonal Antibody (Product # PA5-27463). Samples were transferred to a membrane and probed with TAGLN Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
