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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- GTX54532 - Provider product page
- Provider
- GeneTex
- Product name
- PAX5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of various cell lysates (75 £gg per lane) using PAX5 antibody at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of Ramos and negative control Jurkat cells using PAX5 antibody at a dilution of 1:100 (green). F-Actin (red) was stained with Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of Raji cells with (right) or without (left) PAX5 antibody at a dilution of 1:100 (green). Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP analysis was performed using 750 ug of Raji lysate and 5 ug of PAX5 antibody. The precipitate was detected by the same antibody with EasyBlot as the secondary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human tonsil tissues with (right) or without (left) PAX5 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of mouse lymph node tissues with (right) or without (right) PAX5 antibody at a dilution of 1:100. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of permeabilized Ramos cells using PAX5 antibody at a dilution of 1:50 (red) or PBS alone (black). 30,000 cells were acquired for analysis.
