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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF7199 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal MyBPC3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse MyBPC3 in Western blots and in direct ELISAs.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Association of cardiac myosin-binding protein-C with the ryanodine receptor channel - putative retrograde regulation?
Stanczyk PJ, Seidel M, White J, Viero C, George CH, Zissimopoulos S, Lai FA
Journal of cell science 2018 Aug 3;131(15)
Journal of cell science 2018 Aug 3;131(15)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse MyBPC3 by Western Blot. Western blot shows lysates of mouse heart tissue (adult) and mouse embryonic heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MyBPC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MyBPC3 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
