Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-22304 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Factor IX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.51 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Generation of an mESC model with a human hemophilia B nonsense mutation via CRISPR/Cas9 technology.
Ma Y, Sun W, Zhao L, Yao M, Wu C, Su P, Yang L, Wang G
Stem cell research & therapy 2022 Jul 26;13(1):353
Stem cell research & therapy 2022 Jul 26;13(1):353
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Factor IX using 30 µg of 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Factor IX polyclonal antibody (Product # PA5-22304) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Factor IX was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX Polyclonal Antibody (Product # PA5 22304) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Factor IX was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX Polyclonal Antibody (Product # PA5 22304) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor IX was performed in paraffin-embedded human colon cancer tissue using Factor IX Polyclonal Antibody (Product # PA5-22304) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor IX was performed in paraffin-embedded rat liver tissue using Factor IX Polyclonal Antibody (Product # PA5-22304) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor IX was performed in paraffin-embedded mouse liver tissue using Factor IX Polyclonal Antibody (Product # PA5-22304) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor IX was performed in paraffin-embedded mouse pancreas tissue using Factor IX Polyclonal Antibody (Product # PA5-22304) at a dilution of 1:500.
