PA5-51430

antibody from Invitrogen Antibodies

Targeting: BCL2L2 BCL-W, KIAA0271, PPP1R51

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51430

Provider

Invitrogen Antibodies

Product name

Bcl-W Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MSIEEKMEAD ARSIYVGNVD YGATAEELEA HFHGCGSVNR VTILCDKFSG HPKGFAYIEF SDKESVRTSL ALDESLFRGR QIKVIPKRTN RPGISTTDRG FPRARYRART TNYNSSRSRF YSGFNSRPRG RVYRGRARAT SWY

Concentration

0.1 mg/mL

References

Submitted references

An Anti-PSMA Immunotoxin Reduces Mcl-1 and Bcl2A1 and Specifically Induces in Combination with the BAD-Like BH3 Mimetic ABT-737 Apoptosis in Prostate Cancer Cells.

Masilamani AP, Dettmer-Monaco V, Monaco G, Cathomen T, Kuckuck I, Schultze-Seemann S, Huber N, Wolf P
Cancers 2020 Jun 22;12(6)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Bcl-W in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cerebral cortex tissue. Samples were probed using a Bcl-W Polyclonal Antibody (Product # PA5-51430).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Bcl-W in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Mouse cerebral cortex tissue. Samples were probed using a Bcl-W Polyclonal Antibody (Product # PA5-51430).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Bcl-W in human testis using a Bcl-W Polyclonal Antibody (Product # PA5-51430) shows strong nuclear positivity in cells in seminiferous ducts.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 The immunotoxin hD7-1(VL-VH)-PE40 induces apoptosis in PSMA-positive cells by reducing the expression of Mcl-1 and Bcl2A1. PC cells were incubated for different time points with 1 nM immunotoxin. After cell lysis, expression of the anti-apoptotic Bcl-2 proteins Bcl-2, Bcl-xl, Mcl-1, Bcl-w, and Bcl2A1, the pro-apoptotic Bcl-2 proteins Bax and Bak, as well as the activation of caspase-3, and -8, and the cleavage of PARP were determined by Western blot. ss-actin was detected as loading control.