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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-353-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-353-C100, RRID:AB_2050812
- Product name
- Bcl-w monoclonal antibody (13F9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein G-affinity purified.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 13F9
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Bim and Bad mediate imatinib-induced killing of Bcr/Abl+ leukemic cells, and resistance due to their loss is overcome by a BH3 mimetic.
Proapoptotic BH3-only proteins trigger membrane integration of prosurvival Bcl-w and neutralize its activity.
Tissue expression and subcellular localization of the pro-survival molecule Bcl-w.
Bcl-w is an important determinant of damage-induced apoptosis in epithelia of small and large intestine.
Kuroda J, Puthalakath H, Cragg MS, Kelly PN, Bouillet P, Huang DC, Kimura S, Ottmann OG, Druker BJ, Villunger A, Roberts AW, Strasser A
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 3;103(40):14907-12
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 3;103(40):14907-12
Proapoptotic BH3-only proteins trigger membrane integration of prosurvival Bcl-w and neutralize its activity.
Wilson-Annan J, O'Reilly LA, Crawford SA, Hausmann G, Beaumont JG, Parma LP, Chen L, Lackmann M, Lithgow T, Hinds MG, Day CL, Adams JM, Huang DC
The Journal of cell biology 2003 Sep 1;162(5):877-87
The Journal of cell biology 2003 Sep 1;162(5):877-87
Tissue expression and subcellular localization of the pro-survival molecule Bcl-w.
O'Reilly LA, Print C, Hausmann G, Moriishi K, Cory S, Huang DC, Strasser A
Cell death and differentiation 2001 May;8(5):486-94
Cell death and differentiation 2001 May;8(5):486-94
Bcl-w is an important determinant of damage-induced apoptosis in epithelia of small and large intestine.
Pritchard DM, Print C, O'Reilly L, Adams JM, Potten CS, Hickman JA
Oncogene 2000 Aug 10;19(34):3955-9
Oncogene 2000 Aug 10;19(34):3955-9
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- Enzo Life Sciences (provider)
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- Experimental details
- Immunoprecipitation/Western blot showing the detection of Bcl-w in human and mouse cell lines. Lysates from 107 cells were immunoprecipitated by clone 13F9 and the fractionated proteins were then blotted with the same antibody. Hsp-70 expression was used as an equal loading control, and 293T cells transiently transfected with a human bcl-w expression construct were used as a positive control.
