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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- bs-5251R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-5251R, RRID:AB_11113761
- Product name
- CHEK1(Ser317) Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser317
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Submitted references Presynaptic localization of GluK5 in rod photoreceptors suggests a novel function of high affinity glutamate receptors in the mammalian retina.
Haumann I, Junghans D, Anstötz M, Frotscher M
PloS one 2017;12(2):e0172967
PloS one 2017;12(2):e0172967
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Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti phospho-CHEK1(Ser317) Polyclonal Antibody, Unconjugated (bs-5251R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 40kD. Observed band size: 35kD, 52kD.
- Sample type
- Rat
- Other comments
- Brain
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-phospho-CHEK1(Ser317) Polyclonal Antibody, Unconjugated (bs-5251R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Mouse
- Other comments
- Intestine
