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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB0440 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0440, RRID:AB_982450
- Product name
- CHEK1 (phospho S280) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase.
Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains.
Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1.
Krämer A, Mailand N, Lukas C, Syljuåsen RG, Wilkinson CJ, Nigg EA, Bartek J, Lukas J
Nature cell biology 2004 Sep;6(9):884-91
Nature cell biology 2004 Sep;6(9):884-91
Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains.
Ng CP, Lee HC, Ho CW, Arooz T, Siu WY, Lau A, Poon RY
The Journal of biological chemistry 2004 Mar 5;279(10):8808-19
The Journal of biological chemistry 2004 Mar 5;279(10):8808-19
Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1.
Xu X, Lee J, Stern DF
The Journal of biological chemistry 2004 Aug 13;279(33):34091-4
The Journal of biological chemistry 2004 Aug 13;279(33):34091-4
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The CHEK1 (phospho S280) polyclonal antibody (Cat # PAB0440) is used in Western blot to detect Phospho-CHK1-S280 in mouse brain tissue lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The CHEK1 (phospho S280) polyclonal antibody (Cat # PAB0440) is used in Western blot to detect Phospho-CHK1-S280 in, from left to right, A2058, Ramos, Jurkat, HL-60, and Hela cell lysates.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CHEK1 (phospho S280) polyclonal antibody (Cat # PAB0440) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
