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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- SM6011S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM6011S, RRID:AB_1614513
- Product name
- anti Alpha-crystallin B chain
- Antibody type
- Monoclonal
- Antigen
- Recombinant human α-Crystallin B (1-175 aa) purified from E. coli
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2E8
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Recombinant α-crystallin A (αA) and the extract of mouse eye (ME) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human α-crystallin B antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Recombinant crystallin alpha A (alphaA) and the extract of mouse eye (ME) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human crystallin alpha B antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
