MAB3286

antibody from R&D Systems

Targeting: HSP90AA1 FLJ31884, Hsp89, Hsp90, HSP90N, HSPC1, HSPCA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3286

Provider

R&D Systems

Product name

Human/Mouse/Rat HSP90 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human, mouse and rat HSP90 in direct ELISAs and Western blots. Reacts with both recombinant human HSP90 alpha and HSP90 beta isoforms in direct ELISAs and Western blots.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P08238


Isotype

IgG

Antibody clone number

341320

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4⁺ T cell homeostasis.

Borlido J, Sakuma S, Raices M, Carrette F, Tinoco R, Bradley LM, D'Angelo MA
Nature immunology 2018 Jun;19(6):594-605

---

Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex.

Raices M, Bukata L, Sakuma S, Borlido J, Hernandez LS, Hart DO, D'Angelo MA
Developmental cell 2017 Jun 5;41(5):540-554.e7

---

Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori.

Yao Y, Wu J, Gu T, Cheng Y, Li G
American journal of translational research 2016;8(10):4184-4194

---

Mutation in integrin-linked kinase (ILK(R211A)) and heat-shock protein 70 comprise a broadly cardioprotective complex.

Traister A, Walsh M, Aafaqi S, Lu M, Dai X, Henkleman MR, Momen A, Zhou YQ, Husain M, Arab S, Piran S, Hannigan G, Coles JG
PloS one 2013;8(11):e77331

---

Glucocorticoids enhance CD163 expression in placental Hofbauer cells.

Tang Z, Niven-Fairchild T, Tadesse S, Norwitz ER, Buhimschi CS, Buhimschi IA, Guller S
Endocrinology 2013 Jan;154(1):471-82

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat HSP90 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HSP90 alpha and HSP90 beta (5 ng/lane) were included. A specific band for HSP90 was detected at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Rat HSP90 by Simple Western^TM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP90 at approximately 95 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.