NB100-1972

antibody from Novus Biologicals

Targeting: HSP90AA1 FLJ31884, Hsp89, Hsp90, HSP90N, HSPC1, HSPCA

Immunocytochemistry

Antibody data

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Validation data

Product number: NB100-1972 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB100-1972, RRID:AB_2120909
Product name: Mouse Monoclonal HSP90 Antibody
Antibody type: Monoclonal
Description: Protein G purified. Recognizes both Hsp90 alpha and beta. It reacts with the rodent glucocorticoid receptor and with proliferation potential proteins (P2PS) with apparent molecular masses of 30-40 kDa.
Reactivity: Human, Mouse, Rat, Bacteria, Canine, Chicken/Avian, Guinea Pig, Porcine, Rabbit, Sheep, Simian
Host: Mouse
Antigen sequence: The epitope of this antibody has be en mapped to amino acid residues 60 4-697 of the human Hsp90 sequence.
Isotype: IgG
Vial size: 0.1 mg
Concentration: 1.0 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

HSP90AA1 protein structure - NB100-1972 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the Alexa Fluor 405 conjugated HSP90 antibody, NB100-1972AF405 (purple) and fluorescence minus one control (orange). Staining of HSP90 in Primary CD4+ T-cells derived from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the DyLight 488 conjugate of NB100-1972. Staining of 106 Jurkat cells using HSP90 mAb (AC88), DyLight 488 Conjugate at a concentration of 50ug/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - Analysis using the PE conjugate of NB100-1972. Staining of 10^6 Jurkat cells using HSP90 mAb (AC88), R-Phycoerythrin Conjugate at a concentration of 10ug/mL

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on HeLa cells with HSP90 Antibody (AC88) NB100-1972AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on Jurkat cells with HSP90 Antibody (AC88) NB100-1972AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on HeLa cells with HSP90 Antibody (AC88) NB100-1972PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE format of this antibody.