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LearnMAB1092
antibody from Abnova Corporation
Targeting: HSP90AA1
FLJ31884, Hsp89, Hsp90, HSP90N, HSPC1, HSPCA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB1092 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1092, RRID:AB_1576107
- Product name
- HSP90AA1 monoclonal antibody, clone 4F10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant HSP90AA1.
- Isotype
- IgG
- Antibody clone number
- 4F10
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mass spectrometric/bioinformatic identification of a protein subset that characterizes the cellular activity of anticancer peptides.
Increased proliferation of B cells and auto-immunity in mice lacking protein kinase Cdelta.
Heat shock protein 90 mediates the balance of nitric oxide and superoxide anion from endothelial nitric-oxide synthase.
Quantitation and intracellular localization of the 85K heat shock protein by using monoclonal and polyclonal antibodies.
Genovese F, Gualandi A, Taddia L, Marverti G, Pirondi S, Marraccini C, Perco P, Pelà M, Guerrini R, Amoroso MR, Esposito F, Martello A, Ponterini G, D'Arca D, Costi MP
Journal of proteome research 2014 Nov 7;13(11):5250-61
Journal of proteome research 2014 Nov 7;13(11):5250-61
Increased proliferation of B cells and auto-immunity in mice lacking protein kinase Cdelta.
Miyamoto A, Nakayama K, Imaki H, Hirose S, Jiang Y, Abe M, Tsukiyama T, Nagahama H, Ohno S, Hatakeyama S, Nakayama KI
Nature 2002 Apr 25;416(6883):865-9
Nature 2002 Apr 25;416(6883):865-9
Heat shock protein 90 mediates the balance of nitric oxide and superoxide anion from endothelial nitric-oxide synthase.
Pritchard KA Jr, Ackerman AW, Gross ER, Stepp DW, Shi Y, Fontana JT, Baker JE, Sessa WC
The Journal of biological chemistry 2001 May 25;276(21):17621-4
The Journal of biological chemistry 2001 May 25;276(21):17621-4
Quantitation and intracellular localization of the 85K heat shock protein by using monoclonal and polyclonal antibodies.
Lai BT, Chin NW, Stanek AE, Keh W, Lanks KW
Molecular and cellular biology 1984 Dec;4(12):2802-10
Molecular and cellular biology 1984 Dec;4(12):2802-10
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of cell lysates of HeLa (each 20 ug) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with HSP90AA1 monoclonal antibody , clone 4F10 (1 : 1000) (Cat # MAB1092). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Paraffin embedded sections of human urothelium were incubated with HSP90AA1 monoclonal antibody, clone 4F10 (Cat # MAB1092) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
