ADI-SPA-835-F

antibody from Enzo Life Sciences

Targeting: HSP90AA1 FLJ31884, Hsp89, Hsp90, HSP90N, HSPC1, HSPCA

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ADI-SPA-835-F

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-SPA-835-F, RRID:AB_11181205

Product name

HSP90 monoclonal antibody (16F1)

Antibody type

Monoclonal

Antigen

Purified from natural sources

Description

Protein G affinity purified.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus

Host

Rat

Isotype

IgG

Antibody clone number

16F1

Vial size

200 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell Lines.

Clemente-Vicario F, Alvarez CE, Rowell JL, Roy S, London CA, Kisseberth WC, Lorch G
PloS one 2015;10(11):e0142007

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Identification and quantitative analysis of cellular proteins affected by treatment with withaferin a using a SILAC-based proteomics approach.

Narayan M, Seeley KW, Jinwal UK
Journal of ethnopharmacology 2015 Dec 4;175:86-92

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Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain.

Pearce MM, Spartz EJ, Hong W, Luo L, Kopito RR
Nature communications 2015 Apr 13;6:6768

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The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling.

Wu L, Wang C, Boisson B, Misra S, Rayman P, Finke JH, Puel A, Casanova JL, Li X
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1590-9

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The psoriasis-associated D10N variant of the adaptor Act1 with impaired regulation by the molecular chaperone hsp90.

Wang C, Wu L, Bulek K, Martin BN, Zepp JA, Kang Z, Liu C, Herjan T, Misra S, Carman JA, Gao J, Dongre A, Han S, Bunting KD, Ko JS, Xiao H, Kuchroo VK, Ouyang W, Li X
Nature immunology 2013 Jan;14(1):72-81

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Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase.

Hasham MG, Snow KJ, Donghia NM, Branca JA, Lessard MD, Stavnezer J, Shopland LS, Mills KD
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2374-82

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Methylglyoxal alters the function and stability of critical components of the protein quality control.

Bento CF, Marques F, Fernandes R, Pereira P
PloS one 2010 Sep 24;5(9):e13007

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Enhanced CD95-mediated apoptosis contributes to radiation hypersensitivity of NBS lymphoblasts.

Sagan D, Mörtl S, Müller I, Eckardt-Schupp F, Eichholtz-Wirth H
Apoptosis : an international journal on programmed cell death 2007 Apr;12(4):753-67

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of HSP90: Lane 1: MW marker, Lane 2: HSP90 native protein (ADI-SPP-770), Lane 3: HSP90b recombinant protein (Prod. No. ADI-SPP-772), Lane 4: HSP90alpha recombinant protein (Prod. No. ADI-SPP-776), Lane 5: HeLa (HS), Lane 6: L-929 (HS), Lane 7: Rat-2 (HS), Lane 8:RK-13 (HS).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis of human spleen tissue stained with HSP90, mAb (16F1) at 10µg/ml.