- NB120-2928 - Novus Biologicals HSP90AA1 antibody

Submitted references Tumor-targeted SN38 inhibits growth of early stage non-small cell lung cancer (NSCLC) in a KRas/p53 transgenic mouse model.

Deneka AY, Haber L, Kopp MC, Gaponova AV, Nikonova AS, Golemis EA
PloS one 2017;12(4):e0176747

Inhibiting heat shock protein 90 (HSP90) limits the formation of liver cysts induced by conditional deletion of Pkd1 in mice.

Smithline ZB, Nikonova AS, Hensley HH, Cai KQ, Egleston BL, Proia DA, Seeger-Nukpezah T, Golemis EA
PloS one 2014;9(12):e114403

Inhibiting the HSP90 chaperone slows cyst growth in a mouse model of autosomal dominant polycystic kidney disease.

Seeger-Nukpezah T, Proia DA, Egleston BL, Nikonova AS, Kent T, Cai KQ, Hensley HH, Ying W, Chimmanamada D, Serebriiskii IG, Golemis EA
Proceedings of the National Academy of Sciences of the United States of America 2013 Jul 30;110(31):12786-91

KU135, a novel novobiocin-derived C-terminal inhibitor of the 90-kDa heat shock protein, exerts potent antiproliferative effects in human leukemic cells.

Shelton SN, Shawgo ME, Matthews SB, Lu Y, Donnelly AC, Szabla K, Tanol M, Vielhauer GA, Rajewski RA, Matts RL, Blagg BS, Robertson JD
Molecular pharmacology 2009 Dec;76(6):1314-22

A central role for the Hsp90.Cdc37 molecular chaperone module in interleukin-1 receptor-associated-kinase-dependent signaling by toll-like receptors.

De Nardo D, Masendycz P, Ho S, Cross M, Fleetwood AJ, Reynolds EC, Hamilton JA, Scholz GM
The Journal of biological chemistry 2005 Mar 18;280(11):9813-22

Zonal expression of endothelial nitric oxide synthase in sheep and rhesus adrenal cortex.

Peterson JK, Moran F, Conley AJ, Bird IM
Endocrinology 2001 Dec;142(12):5351-63

Localization and characterization of the 86- and 84-kDa heat shock proteins in Hepa 1c1c7 cells.

Perdew GH, Hord N, Hollenback CE, Welsh MJ
Experimental cell research 1993 Dec;209(2):350-6

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HSP90 alpha Antibody [NB120-2928] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of epidermoid carcinoma (A431) cells using Heat Shock Protein 86 Polyclonal Antibody. Decreasing amounts of A431 whole cell lysates were probed with PA3-013 at a dilution of 1:2000 followed by an anti-rabbit IgG secondary antibody and SuperSignal West Pico Chemiluminescent Substrate. Lanes 1 and 2 are control samples of purified, recombinant Hsp86 (rHsp86) and Hsp84 (rHsp84), respectively. Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HSP90 alpha Antibody [NB120-2928] - Figure 1 shows a Western blot of mouse HSP84 and HSP86 using NB120-2928 and NB120-2927 respectively.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoprecipitation: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein (HSP86) was performed on HeLa cells. Antigen-antibody complexes formed by incubating 500ug whole cell lysate with 2ug of HSP86 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C. The membrane was washed in TBST, and probed with Clean-Blot IP detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Analysis of differentiated colon adenocarcinoma from human colon tissue sections. To expose target proteins, heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95C. Following antigen retrieval, tissues were blocked in 3% BSA in PBST for 30 minutes at room temperature and then probed with a Heat Shock Protein 86 (Hsp86) polyclonal antibody at a dilution of 1:100 for 1 hour in a humidified chamber (right panel). As a negative control, the primary antibody was eliminated from the staining procedure (left panel). Tissues were washed extensively with PBS/0.025% Tween-20 and endogenous peroxidase activity quenched with Peroxidase Suppressor for 30 minutes at room temperature. Detection was performed using an HRP-conjugated goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:250 followed by colorimetric detection using Metal Enhanced DAB Substrate Kit. Tissues were counterstained with hematoxylin and prepped for mouting. Images were taken on a Zeiss Axiovision microscope at 40X magnification (x1.6 Optovar).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

NB120-2928

Antibody data