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- Antigen structure
- References [39]
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- Product number
- BBA16 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal E-Selectin/CD62E Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects E-Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM-1, L-Selectin, P-Selectin, or VCAM-1.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 200 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-κB Signaling.
Pinocembrin protects endothelial cells from oxidized LDL-induced injury.
Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death.
Bordetella pertussis Adenylate Cyclase Toxin Disrupts Functional Integrity of Bronchial Epithelial Layers.
A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.
A modular platform for targeted RNAi therapeutics.
Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in vivo and in vitro.
Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression.
The Abl-1 Kinase is Dispensable for NK Cell Inhibitory Signalling and is not Involved in Murine NK Cell Education.
Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture.
Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles.
Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs Via an Intrinsic Mitochondria-Dependent Pathway.
Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T cells and T-bet/GATA-3 expression in NOD mice.
Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system.
Purinergic Receptor Transactivation by the β2-Adrenergic Receptor Increases Intracellular Ca2+ in Nonexcitable Cells.
Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine.
Blockade of interleukin-6 receptor in the periphery promotes rapid and sustained antidepressant actions: a possible role of gut-microbiota-brain axis.
Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model.
Amine oxidase activity regulates the development of pulmonary fibrosis.
AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis.
Three-Dimensional Coculture Model to Analyze the Cross Talk Between Endothelial and Smooth Muscle Cells.
Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis.
Soluble ST2 predicts 1-year outcome in patients undergoing transcatheter aortic valve implantation.
Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment.
Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation.
Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model.
Plasma membrane Toll-like receptor activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of interferon-β.
Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress.
IL-17RB+ granulocytes are associated with airflow obstruction in asthma.
Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
The role of endothelial cell reactive antibodies in peripheral arterial disease.
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Differential effects of orbital and laminar shear stress on endothelial cells.
Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction.
Seigner J, Junker-Samek M, Plaza A, D'Urso G, Masullo M, Piacente S, Holper-Schichl YM, de Martin R
Frontiers in pharmacology 2019;10:289
Frontiers in pharmacology 2019;10:289
Pinocembrin protects endothelial cells from oxidized LDL-induced injury.
Su Q, Sun Y, Ye Z, Yang H, Kong B, Li L
Cytokine 2018 Nov;111:475-480
Cytokine 2018 Nov;111:475-480
Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death.
Wan H, Tang B, Liao X, Zeng Q, Zhang Z, Liao L
Cell death and differentiation 2018 May;25(5):904-917
Cell death and differentiation 2018 May;25(5):904-917
Bordetella pertussis Adenylate Cyclase Toxin Disrupts Functional Integrity of Bronchial Epithelial Layers.
Hasan S, Kulkarni NN, Asbjarnarson A, Linhartova I, Osicka R, Sebo P, Gudmundsson GH
Infection and immunity 2018 Mar;86(3)
Infection and immunity 2018 Mar;86(3)
A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.
Howley BV, Link LA, Grelet S, El-Sabban M, Howe PH
Oncogene 2018 Mar;37(10):1308-1325
Oncogene 2018 Mar;37(10):1308-1325
A modular platform for targeted RNAi therapeutics.
Kedmi R, Veiga N, Ramishetti S, Goldsmith M, Rosenblum D, Dammes N, Hazan-Halevy I, Nahary L, Leviatan-Ben-Arye S, Harlev M, Behlke M, Benhar I, Lieberman J, Peer D
Nature nanotechnology 2018 Mar;13(3):214-219
Nature nanotechnology 2018 Mar;13(3):214-219
Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in vivo and in vitro.
Zhang H, Fang X, Huang D, Luo Q, Zheng M, Wang K, Cao L, Yin Z
Molecular medicine reports 2018 Jan;17(1):264-272
Molecular medicine reports 2018 Jan;17(1):264-272
Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression.
Donnelly C, Dykstra B, Mondal N, Huang J, Kaskow BJ, Griffin R, Sackstein R, Baecher-Allan C
Scientific reports 2018 Jan 11;8(1):420
Scientific reports 2018 Jan 11;8(1):420
The Abl-1 Kinase is Dispensable for NK Cell Inhibitory Signalling and is not Involved in Murine NK Cell Education.
Ganesan S, Luu TT, Chambers BJ, Meinke S, Brodin P, Vivier E, Wetzel DM, Koleske AJ, Kadri N, Höglund P
Scandinavian journal of immunology 2017 Sep;86(3):135-142
Scandinavian journal of immunology 2017 Sep;86(3):135-142
Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture.
Cheng X, Xie B, Qi J, Zhao X, Zhang Z, Qiu M, Yang J
Developmental neurobiology 2017 Sep;77(8):907-916
Developmental neurobiology 2017 Sep;77(8):907-916
Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles.
Bui TMT, Nguyễn KX, Karata A, Ferré P, Trần MT, Wakai T, Funahashi H
Reproduction, fertility, and development 2017 Sep;29(10):1902-1909
Reproduction, fertility, and development 2017 Sep;29(10):1902-1909
Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs Via an Intrinsic Mitochondria-Dependent Pathway.
Wu GJ, Chen JT, Tsai HC, Chen TL, Liu SH, Chen RM
Journal of cellular biochemistry 2017 Sep;118(9):2635-2644
Journal of cellular biochemistry 2017 Sep;118(9):2635-2644
Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T cells and T-bet/GATA-3 expression in NOD mice.
Tang A, Li C, Chen Z, Li T
Molecular medicine reports 2017 Oct;16(4):3974-3982
Molecular medicine reports 2017 Oct;16(4):3974-3982
Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system.
Yan K, Shen Y
Molecular medicine reports 2017 Oct;16(4):3965-3973
Molecular medicine reports 2017 Oct;16(4):3965-3973
Purinergic Receptor Transactivation by the β2-Adrenergic Receptor Increases Intracellular Ca2+ in Nonexcitable Cells.
Stallaert W, van der Westhuizen ET, Schönegge AM, Plouffe B, Hogue M, Lukashova V, Inoue A, Ishida S, Aoki J, Le Gouill C, Bouvier M
Molecular pharmacology 2017 May;91(5):533-544
Molecular pharmacology 2017 May;91(5):533-544
Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine.
Ding YF, Wu ZH, Wei YJ, Shu L, Peng YR
Journal of cancer research and clinical oncology 2017 May;143(5):821-834
Journal of cancer research and clinical oncology 2017 May;143(5):821-834
Blockade of interleukin-6 receptor in the periphery promotes rapid and sustained antidepressant actions: a possible role of gut-microbiota-brain axis.
Zhang JC, Yao W, Dong C, Yang C, Ren Q, Ma M, Hashimoto K
Translational psychiatry 2017 May 30;7(5):e1138
Translational psychiatry 2017 May 30;7(5):e1138
Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model.
Sulek JE, Robinson SP, Petrossian AA, Zhou S, Goliadze E, Manjili MH, Toor A, Guruli G
The Prostate 2017 Mar;77(4):361-373
The Prostate 2017 Mar;77(4):361-373
Amine oxidase activity regulates the development of pulmonary fibrosis.
Marttila-Ichihara F, Elima K, Auvinen K, Veres TZ, Rantakari P, Weston C, Miyasaka M, Adams D, Jalkanen S, Salmi M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Jun;31(6):2477-2491
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Jun;31(6):2477-2491
AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis.
Yamaguchi K, Iwamoto H, Horimasu Y, Ohshimo S, Fujitaka K, Hamada H, Mazur W, Kohno N, Hattori N
Respirology (Carlton, Vic.) 2017 Jul;22(5):965-971
Respirology (Carlton, Vic.) 2017 Jul;22(5):965-971
Three-Dimensional Coculture Model to Analyze the Cross Talk Between Endothelial and Smooth Muscle Cells.
Ganesan MK, Finsterwalder R, Leb H, Resch U, Neumüller K, de Martin R, Petzelbauer P
Tissue engineering. Part C, Methods 2017 Jan;23(1):38-49
Tissue engineering. Part C, Methods 2017 Jan;23(1):38-49
Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis.
Zhang Y, Wang Z, Liu J, Zhang S, Fei J, Li J, Zhang T, Wang J, Park PW, Chen Y
Journal of cellular and molecular medicine 2017 Jan;21(1):13-25
Journal of cellular and molecular medicine 2017 Jan;21(1):13-25
Soluble ST2 predicts 1-year outcome in patients undergoing transcatheter aortic valve implantation.
Wernly B, Lichtenauer M, Jirak P, Eder S, Reiter C, Kammler J, Kypta A, Jung C, Franz M, Hoppe UC, Landmesser U, Figulla HR, Lauten A
European journal of clinical investigation 2017 Feb;47(2):149-157
European journal of clinical investigation 2017 Feb;47(2):149-157
Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment.
Vernot JP, Bonilla X, Rodriguez-Pardo V, Vanegas NP
International journal of molecular sciences 2017 Feb 14;18(2)
International journal of molecular sciences 2017 Feb 14;18(2)
Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation.
Lin XL, Lv JJ, Lv J, Di CX, Zhang YJ, Zhou T, Liu JL, Xia ZW
Allergy 2017 Dec;72(12):1972-1987
Allergy 2017 Dec;72(12):1972-1987
Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model.
Ratanavaraporn J, Soontornvipart K, Shuangshoti S, Shuangshoti S, Damrongsakkul S
Inflammopharmacology 2017 Apr;25(2):211-221
Inflammopharmacology 2017 Apr;25(2):211-221
Plasma membrane Toll-like receptor activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of interferon-β.
Boye L, Welsby I, Lund LD, Goriely S, Frøkiaer H
Immunology 2016 Nov;149(3):329-342
Immunology 2016 Nov;149(3):329-342
Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress.
Idris-Khodja N, Ouerd S, Mian MOR, Gornitsky J, Barhoumi T, Paradis P, Schiffrin EL
American journal of hypertension 2016 Nov 1;29(11):1245-1251
American journal of hypertension 2016 Nov 1;29(11):1245-1251
IL-17RB+ granulocytes are associated with airflow obstruction in asthma.
Li L, Lukacs NW, Schaller MA, Petersen B, Baptist AP
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 2016 Dec;117(6):674-679
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 2016 Dec;117(6):674-679
Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
Pezeshkian B, Donnelly C, Tamburo K, Geddes T, Madlambayan GJ
PloS one 2013;8(4):e60823
PloS one 2013;8(4):e60823
Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
Schellerer VS, Croner RS, Weinländer K, Hohenberger W, Stürzl M, Naschberger E
Laboratory investigation; a journal of technical methods and pathology 2007 Nov;87(11):1159-70
Laboratory investigation; a journal of technical methods and pathology 2007 Nov;87(11):1159-70
A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
Developmental biology 2006 Oct 1;298(1):107-17
Developmental biology 2006 Oct 1;298(1):107-17
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
The Journal of experimental medicine 2006 Nov 27;203(12):2763-77
The Journal of experimental medicine 2006 Nov 27;203(12):2763-77
Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
Blood 2006 Mar 15;107(6):2252-61
Blood 2006 Mar 15;107(6):2252-61
The role of endothelial cell reactive antibodies in peripheral arterial disease.
Armitage JD, Lindsey NJ, Homer-Vanniasinkam S
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 Feb;31(2):170-5
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 Feb;31(2):170-5
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damås JK
Blood 2006 Aug 1;108(3):928-35
Blood 2006 Aug 1;108(3):928-35
Differential effects of orbital and laminar shear stress on endothelial cells.
Dardik A, Chen L, Frattini J, Asada H, Aziz F, Kudo FA, Sumpio BE
Journal of vascular surgery 2005 May;41(5):869-80
Journal of vascular surgery 2005 May;41(5):869-80
Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
Helanterä I, Loginov R, Koskinen P, Törnroth T, Grönhagen-Riska C, Lautenschlager I
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2005 Apr;20(4):790-6
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2005 Apr;20(4):790-6
Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction.
Rémy-Zolghadri M, Laganière J, Oligny JF, Germain L, Auger FA
Journal of vascular surgery 2004 Mar;39(3):613-20
Journal of vascular surgery 2004 Mar;39(3):613-20
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- E-Selectin/CD62E in Human Brain. E-Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
