BBA1

antibody from R&D Systems

Targeting: SELE CD62E, ELAM, ELAM1, ESEL

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

BBA1

Provider

R&D Systems

Product name

Human E-Selectin/P-Selectin (CD62E/P) Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human E-Selectin/P-Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

BBIG-E6 (13D5)

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction.

Belcher JD, Chen C, Nguyen J, Abdulla F, Zhang P, Nguyen H, Nguyen P, Killeen T, Miescher SM, Brinkman N, Nath KA, Steer CJ, Vercellotti GM
PloS one 2018;13(4):e0196455

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Self-medication by orang-utans (Pongo pygmaeus) using bioactive properties of Dracaena cantleyi.

Morrogh-Bernard HC, Foitová I, Yeen Z, Wilkin P, de Martin R, Rárová L, Doležal K, Nurcahyo W, Olšanský M
Scientific reports 2017 Nov 30;7(1):16653

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Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells.

Rouger C, Pagie S, Derbré S, Le Ray AM, Richomme P, Charreau B
PloS one 2016;11(12):e0167361

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SDF-1α-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia.

Liu ZJ, Tian R, Li Y, Zhang L, Shao H, Yang C, Velazquez OC
Scientific reports 2016 Oct 7;6:34416

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West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.

Roe K, Orillo B, Verma S
PloS one 2014;9(7):e102598

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Functional diversity of human vaginal APC subsets in directing T-cell responses.

Duluc D, Gannevat J, Anguiano E, Zurawski S, Carley M, Boreham M, Stecher J, Dullaers M, Banchereau J, Oh S
Mucosal immunology 2013 May;6(3):626-38

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Activation of primary porcine endothelial cells induces release of porcine endogenous retroviruses.

Cunningham DA, Dos Santos Cruz GJ, Fernández-Suárez XM, Whittam AJ, Herring C, Copeman L, Richards A, Langford GA
Transplantation 2004 Apr 15;77(7):1071-9

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Activation of primary porcine endothelial cells induces release of porcine endogenous retroviruses.

Cunningham DA, Dos Santos Cruz GJ, Fernández-Suárez XM, Whittam AJ, Herring C, Copeman L, Richards A, Langford GA
Transplantation 2004 Apr 15;77(7):1071-9

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells. E-Selectin (CD62E)/P-Selectin (CD62P) was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin (CD62E)/P-Selectin (CD62P) Monoclonal Antibody (Catalog # BBA1) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.