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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 14-0627-37 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The P2H3 monoclonal antibody reacts with human CD62E, a 97-115 kDa member of the selectin family. CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFalpha and IL-1beta. Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium.
- Antibody clone number
- P2H3
- Concentration
- 0.5 mg/mL
Submitted references Surgical and intravital microscopy protocol to image Trypanosoma brucei-host interactions in live rodent models.
Organotypic endothelial adhesion molecules are key for Trypanosoma brucei tropism and virulence.
A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy.
Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils.
Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion.
Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.
De Niz M, Figueiredo LM
STAR protocols 2022 Jun 17;3(2):101450
STAR protocols 2022 Jun 17;3(2):101450
Organotypic endothelial adhesion molecules are key for Trypanosoma brucei tropism and virulence.
De Niz M, Brás D, Ouarné M, Pedro M, Nascimento AM, Henao Misikova L, Franco CA, Figueiredo LM
Cell reports 2021 Sep 21;36(12):109741
Cell reports 2021 Sep 21;36(12):109741
A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy.
Chidiac R, Abedin M, Macleod G, Yang A, Thibeault PE, Blazer LL, Adams JJ, Zhang L, Roehrich H, Jo HN, Seshagiri S, Sidhu SS, Junge HJ, Angers S
EMBO molecular medicine 2021 Jul 7;13(7):e13977
EMBO molecular medicine 2021 Jul 7;13(7):e13977
Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils.
Manriquez V, Nivoit P, Urbina T, Echenique-Rivera H, Melican K, Fernandez-Gerlinger MP, Flamant P, Schmitt T, Bruneval P, Obino D, Duménil G
Nature communications 2021 Jul 27;12(1):4547
Nature communications 2021 Jul 27;12(1):4547
Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion.
Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A Jr, Neelamegham S
Scientific reports 2016 Jul 26;6:30392
Scientific reports 2016 Jul 26;6:30392
Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.
An N, Andrukhov O, Tang Y, Falkensammer F, Bantleon HP, Ouyang X, Rausch-Fan X
PloS one 2014;9(5):e96942
PloS one 2014;9(5):e96942
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CD62E (E-selectin) Monoclonal Antibody (Product # 14-0627-82) and 67, 115 kDa bands corresponding to CD62E were observed across the tissues tested. Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Spleen (Lane 2) and Mouse White Adipose (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of TNF alpha-stimulated Human Umbilical Vein Endothelial Cells (HUVEC) with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD62E (E- Selectin) Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Effect of nicotine on the P. gingivalis LPS-induced protein expression of pro-inflammatory mediators in HUVECs. HUVECs were stimulated by P. gingivalis LPS in the presence or absence of nicotine (10 uM-10 mM) for 4, 24, and 72 h. After stimulation, the surface expression levels of ICAM-1 (A), VCAM-1 (B), and E-selectin (C) were measured by flow cytometry, and the quantity of MCP-1 (D) and IL-8 (E) in conditioned media was measured by ELISA. Each value represents mean +-SD of three independent assays. Non-stimulated HUVECs were used as a control. The protein expression levels of pro-inflammatory mediators were not analyzed after stimulation with 10-mM nicotine for 24 and 72 h because the cells were not viable. * - significantly different between groups, p
