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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NBP1-45545 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-45545, RRID:AB_10009002
- Product name
- Rabbit Polyclonal E-Selectin/CD62E Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Atheroprone flow activates inflammation via endothelial ATP-dependent P2X7-p38 signalling.
Endothelial HuR deletion reduces the expression of proatherogenic molecules and attenuates atherosclerosis.
Irradiation with 310 nm and 340 nm ultraviolet light-emitting-diodes can improve atopic dermatitis-like skin lesions in NC/Nga mice.
Effect of a 308-nm excimer laser on atopic dermatitis-like skin lesions in NC/Nga mice.
Therapeutic effects of full spectrum light on the development of atopic dermatitis-like lesions in NC/Nga mice.
Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells.
Green JP, Souilhol C, Xanthis I, Martinez-Campesino L, Bowden NP, Evans PC, Wilson HL
Cardiovascular research 2018 Feb 1;114(2):324-335
Cardiovascular research 2018 Feb 1;114(2):324-335
Endothelial HuR deletion reduces the expression of proatherogenic molecules and attenuates atherosclerosis.
Fu X, Zhai S, Yuan J
International immunopharmacology 2018 Dec;65:248-255
International immunopharmacology 2018 Dec;65:248-255
Irradiation with 310 nm and 340 nm ultraviolet light-emitting-diodes can improve atopic dermatitis-like skin lesions in NC/Nga mice.
Kwon TR, Kim JH, Hong JY, Seok J, Kim JM, Bak DH, Choi MJ, Mun SK, Kim CW, Kim BJ
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2018 Aug 8;17(8):1127-1135
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2018 Aug 8;17(8):1127-1135
Effect of a 308-nm excimer laser on atopic dermatitis-like skin lesions in NC/Nga mice.
Oh CT, Kwon TR, Seok J, Choi EJ, Kim SR, Jang YJ, Mun SK, Kim CW, Lee S, Lee J, Kim MN, Choi SY, Kim BJ
Lasers in surgery and medicine 2016 Aug;48(6):629-37
Lasers in surgery and medicine 2016 Aug;48(6):629-37
Therapeutic effects of full spectrum light on the development of atopic dermatitis-like lesions in NC/Nga mice.
Kwon TR, Mun SK, Oh CT, Hong H, Choi YS, Kim BJ, Kim BJ
Photochemistry and photobiology 2014 Sep-Oct;90(5):1160-9
Photochemistry and photobiology 2014 Sep-Oct;90(5):1160-9
Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells.
Jung CH, Lee MJ, Kang YM, Lee YL, Yoon HK, Kang SW, Lee WJ, Park JY
Cardiovascular diabetology 2014 Feb 12;13:41
Cardiovascular diabetology 2014 Feb 12;13:41
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: E-Selectin/CD62E Antibody [NBP1-45545] - Simple Western lane view shows a specific band for E Selectin/CD62E in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: E-Selectin/CD62E Antibody [NBP1-45545] - Jurkat cell lysate (80 ug) loaded Blocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST E-Selectin Polyclonal Antibody used at 1:1000 dilution - O/N at 4 degrees C. Goat Anti-Rabbit IgG peroxidase conjugated used at 1:10,000 dilution - 1 hr at RT
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody [NBP1-45545] - Staining of aorta paraffin section from obese diabetic mice. Staining of Endothelial cell lair has been observed.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody [NBP1-45545] - Staining of aorta paraffin section from obese diabetic mice. Staining of Endothelial cell lair has been observed.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody [NBP1-45545] - Formalin fixed, paraffin-embedded tissue after heat induced antigen retrieval. 5 ug/ml of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
