Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF4958 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Plexin B3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Plexin B3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Plexin B3 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Plexin B3 by Western Blot. Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4958) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Human Plexin B3 (Catalog # 4958-PC) (1 ng) was included. A specific band for Plexin B3 was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Plexin B3 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Sheep Anti-Human Plexin B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4958, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).