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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-SA227-0025 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-SA227-0025, RRID:AB_2052072
- Product name
- nNOS polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Protein A-affinity purified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Vial size
- 25 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, Sonobe T, Inagaki T, Fujii Y, Yamaguchi R, Wingenfeld L, Umetani K, Shimosawa T, Yoshida K, Uemura K, Pearson JT, Shirai M
PloS one 2015;10(7):e0131923
PloS one 2015;10(7):e0131923
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot analysis of nNOS, pAb (Prod. No. BML-SA227) using whole cell extracts from (1) rat brain (BML-SW103), (2) mouse brain (Prod. No. BML-SW104), and (3,4) 10 ng recombinant rat bNOS (Prod. No. BML-SW109). In lane (4) the antibody was preincubated with Prod. No. BML-SP227 blocking control peptide. The nitrocellulose membrane was probed with Prod. No. BML-SA227 at 5 _g/ml. The secondary antibody was GAR-AP diluted 1/2000 and the blot was developed with BCIP/NBT.
