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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX129058 - Provider product page
- Provider
- GeneTex
- Product name
- eNOS (phospho Ser1177) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Long-Term Administration of Angiotensin (1-7) to db/db Mice Reduces Oxidative Stress Damage in the Kidneys and Prevents Renal Dysfunction.
Preserved Vasoconstriction and Relaxation of Saphenous Vein Grafts Obtained by a No-Touch Technique for Coronary Artery Bypass Grafting.
Papinska AM, Rodgers KE
Oxidative medicine and cellular longevity 2018;2018:1841046
Oxidative medicine and cellular longevity 2018;2018:1841046
Preserved Vasoconstriction and Relaxation of Saphenous Vein Grafts Obtained by a No-Touch Technique for Coronary Artery Bypass Grafting.
Yamada T, Adachi T, Ido Y, Masaki N, Toya T, Uchimuro T, Nishigawa K, Suda H, Osako M, Yamazaki M, Takanashi S, Shimizu H
Circulation journal : official journal of the Japanese Circulation Society 2018 Dec 25;83(1):232-238
Circulation journal : official journal of the Japanese Circulation Society 2018 Dec 25;83(1):232-238
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein by western blot analysis.A. 30 ?g THP-1 whole cell lysate/extract (untreated)B. 30 ?g THP-1 whole cell lysate/extract (100 nM PMA treatment for 6 hr) C. 30 ?g THP-1 whole cell lysate/extract (untreated) D. 30 ?g THP-1 whole cell lysate/extract (100 nM PMA treatment for 24 hr) E. 30 ?g THP-1 whole cell lysate/extract (untreated) F. 30 ?g THP-1 whole cell lysate/extract (100 nM PMA treatment for 48 hr)5% SDS-PAGEeNOS (phospho Ser1177) antibody (GTX129058) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extract B. 30 ?g Raji whole cell lysate/extract C. 30 ?g NCI-H929 whole cell lysate/extract5% SDS-PAGEeNOS (phospho Ser1177) antibody (GTX129058) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) THP-1 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein in Phorbol 12-Myristate 13-Acetate treated samples by Western blot analysis.Upperpanel: eNOS (phospho Ser1177) antibody (GTX100042)Lower panel: Vinculin antibody [N3C1], Internal (GTX109749)A. 30 £gg THP-1 whole cell lysate/extract B. 30 £gg THP-1 whole cell lysate/extract (100 nM Phorbol 12-Myristate 13-Acetate treatment for 24 hr)5 % SDS-PAGEeNOS (phospho Ser1177) antibody (GTX129058) dilution: 1:1000Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:500
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm and Golgi apparatus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: eNOS (phospho Ser1177) protein stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GTX129058) diluted at 1:500.
