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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- ARG51154 - Provider product page
- Provider
- Arigo
- Product name
- anti-eNOS antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from Rat heart, Mouse heart and Rat kidney tissue stained with anti-eNOS antibody ARG51154.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of 1) MEF and 2) HUVEC cell line lysates stained with ARG51154 anti-eNOS antibody at 1:500 dilution.Data provided by customer.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: methanol-fixed HeLa cells stained with anti-eNOS antibody ARG51154.
