Antibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A01604-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-eNOS/NOS3 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for eNOS/NOS3 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Comparison of acute kidney injury following brain death between male and female rats.
Placental expression of striatin & endothelial nitric oxide synthase in women with & without pre-eclampsia.
Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis.
Buxuhuayu decoction accelerates angiogenesis by activating the PI3K-Akt-eNOS signalling pathway in a streptozotocin-induced diabetic ulcer rat model.
Increased secretion of VEGF-C from SiO(2)-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis.
Pioglitazone, a Peroxisome Proliferator-Activated Receptor x03B3; Agonist, Ameliorates Chronic Kidney Disease by Enhancing Antioxidative Capacity and Attenuating Angiogenesis in the Kidney of a 5/6 Nephrectomized Rat Model.
Autologous Bone Marrow Mononuclear Cell Transplantation Delays Progression of Carotid Atherosclerosis in Rabbits.
Fucoidan from Undaria pinnatifida prevents vascular dysfunction through PI3K/Akt/eNOS-dependent mechanisms in the l-NAME-induced hypertensive rat model.
Effects of thyroid hormones on the antioxidative status in the uterus of young adult rats.
Nitric oxide and thyroid hormone receptor alpha 1 contribute to ovarian follicular development in immature hyper- and hypo-thyroid rats.
Protective effects of Big-leaf mulberry and physiological roles of nitric oxide synthases in the testis of mice following water immersion and restraint stress.
Sodium ferulate lowers portal pressure in rats with secondary biliary cirrhosis through the RhoA/Rho-kinase signaling pathway: a preliminary study.
Reproductive toxicity in acrylamide-treated female mice.
Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and soluble guanylyl cyclase α and β subunits in postnatal porcine uteri.
Intermittent maternal hypoxia has an influence on regional expression of endothelial nitric oxide synthase in fetal arteries of rabbits.
Sesamin ameliorates arterial dysfunction in spontaneously hypertensive rats via downregulation of NADPH oxidase subunits and upregulation of eNOS expression.
Endothelial NO synthase and reactive oxygen species mediated effect of simvastatin on vessel structure and function: pleiotropic and dose-dependent effect on tumor vascular stabilization.
Sesamin exerts renoprotective effects by enhancing NO bioactivity in renovascular hypertensive rats fed with high-fat-sucrose diet.
2,3,4',5-tetrahydroxystilbene-2-O-β-D-glucoside inhibits proliferation of vascular smooth muscle cells: involvement of NO/cGMP/PKG pathway.
Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats.
Effects of quercetin on gene and protein expression of NOX and NOS after myocardial ischemia and reperfusion in rabbit.
Sesamin improves endothelial dysfunction in renovascular hypertensive rats fed with a high-fat, high-sucrose diet.
Effects of calcitonin gene-related peptide on the expression and activity of nitric oxide synthase during mandibular bone healing in rabbits: an experimental study.
Protective effects of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-d-glucoside, an active component of Polygonum multiflorum Thunb, on experimental colitis in mice.
Research on the age-related changes in the nitric oxide pathway in the arteries of rats and the intervention effect of dehydroepiandrosterone.
Inhibition by brimonidine of forskolin-induced nitric oxide synthase expression in human ciliary bodies in vitro.
Armstrong-Jr R, Ricardo-da-Silva FY, Vidal-Dos-Santos M, da Anunciação LF, Ottens PJ, Correia CJ, Moreira LFP, Leuvenink HGD, Breithaupt-Faloppa AC
Clinics (Sao Paulo, Brazil) 2023;78:100222
Clinics (Sao Paulo, Brazil) 2023;78:100222
Placental expression of striatin & endothelial nitric oxide synthase in women with & without pre-eclampsia.
Viveka M, Chaturvedula L, Ram AK, Vairappan B
The Indian journal of medical research 2023 Apr;157(4):345-352
The Indian journal of medical research 2023 Apr;157(4):345-352
Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis.
Chen L, Liu D, Mao M, Liu W, Wang Y, Liang Y, Cao W, Zhong X
Molecular nutrition & food research 2022 Nov;66(22):e2200341
Molecular nutrition & food research 2022 Nov;66(22):e2200341
Buxuhuayu decoction accelerates angiogenesis by activating the PI3K-Akt-eNOS signalling pathway in a streptozotocin-induced diabetic ulcer rat model.
Qu K, Cha H, Ru Y, Que H, Xing M
Journal of ethnopharmacology 2021 Jun 12;273:113824
Journal of ethnopharmacology 2021 Jun 12;273:113824
Increased secretion of VEGF-C from SiO(2)-induced pulmonary macrophages promotes lymphangiogenesis through the Src/eNOS pathway in silicosis.
Zhang J, Cui J, Li X, Hao X, Guo L, Wang H, Liu H
Ecotoxicology and environmental safety 2021 Apr 29;218:112257
Ecotoxicology and environmental safety 2021 Apr 29;218:112257
Pioglitazone, a Peroxisome Proliferator-Activated Receptor x03B3; Agonist, Ameliorates Chronic Kidney Disease by Enhancing Antioxidative Capacity and Attenuating Angiogenesis in the Kidney of a 5/6 Nephrectomized Rat Model.
Sun L, Yuan Q, Xu T, Yao L, Feng J, Ma J, Wang L, Lu C, Wang D
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;38(5):1831-40
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;38(5):1831-40
Autologous Bone Marrow Mononuclear Cell Transplantation Delays Progression of Carotid Atherosclerosis in Rabbits.
Cui K, Ma X, Yu L, Jiang C, Fu C, Fu X, Yu X, Huang Y, Hou S, Si C, Chen Z, Yu J, Wan J, Wang J
Molecular neurobiology 2016 Sep;53(7):4387-96
Molecular neurobiology 2016 Sep;53(7):4387-96
Fucoidan from Undaria pinnatifida prevents vascular dysfunction through PI3K/Akt/eNOS-dependent mechanisms in the l-NAME-induced hypertensive rat model.
Li X, Li J, Li Z, Sang Y, Niu Y, Zhang Q, Ding H, Yin S
Food & function 2016 May 18;7(5):2398-408
Food & function 2016 May 18;7(5):2398-408
Effects of thyroid hormones on the antioxidative status in the uterus of young adult rats.
Kong L, Wei Q, Fedail JS, Shi F, Nagaoka K, Watanabe G
The Journal of reproduction and development 2015;61(3):219-27
The Journal of reproduction and development 2015;61(3):219-27
Nitric oxide and thyroid hormone receptor alpha 1 contribute to ovarian follicular development in immature hyper- and hypo-thyroid rats.
Zheng K, Sulieman FJ, Li J, Wei Q, Xu M, Shi F
Reproductive biology 2015 Mar;15(1):27-33
Reproductive biology 2015 Mar;15(1):27-33
Protective effects of Big-leaf mulberry and physiological roles of nitric oxide synthases in the testis of mice following water immersion and restraint stress.
Xu M, Wei Q, Zheng K, Mao D, Zheng Y, Li Y, Shi F
Acta histochemica 2014 Oct;116(8):1323-30
Acta histochemica 2014 Oct;116(8):1323-30
Sodium ferulate lowers portal pressure in rats with secondary biliary cirrhosis through the RhoA/Rho-kinase signaling pathway: a preliminary study.
Wei L, Yang J, Wang M, Xu SN, Liang HM, Zhou Q
International journal of molecular medicine 2014 Nov;34(5):1257-67
International journal of molecular medicine 2014 Nov;34(5):1257-67
Reproductive toxicity in acrylamide-treated female mice.
Wei Q, Li J, Li X, Zhang L, Shi F
Reproductive toxicology (Elmsford, N.Y.) 2014 Jul;46:121-8
Reproductive toxicology (Elmsford, N.Y.) 2014 Jul;46:121-8
Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and soluble guanylyl cyclase α and β subunits in postnatal porcine uteri.
Li Y, Zhou X, Wei QW, Huang RH, Shi FX
Acta histochemica 2014 Apr;116(3):466-73
Acta histochemica 2014 Apr;116(3):466-73
Intermittent maternal hypoxia has an influence on regional expression of endothelial nitric oxide synthase in fetal arteries of rabbits.
Chen YF, Wang ZH, Chen ZK, Lv GR, Ferrari M
Pediatric research 2013 Jun;73(6):706-12
Pediatric research 2013 Jun;73(6):706-12
Sesamin ameliorates arterial dysfunction in spontaneously hypertensive rats via downregulation of NADPH oxidase subunits and upregulation of eNOS expression.
Zhang JX, Yang JR, Chen GX, Tang LJ, Li WX, Yang H, Kong X
Acta pharmacologica Sinica 2013 Jul;34(7):912-20
Acta pharmacologica Sinica 2013 Jul;34(7):912-20
Endothelial NO synthase and reactive oxygen species mediated effect of simvastatin on vessel structure and function: pleiotropic and dose-dependent effect on tumor vascular stabilization.
Chen Y, Zhang S, Peng G, Yu J, Liu T, Meng R, Li Z, Zhao Y, Wu G
International journal of oncology 2013 Apr;42(4):1325-36
International journal of oncology 2013 Apr;42(4):1325-36
Sesamin exerts renoprotective effects by enhancing NO bioactivity in renovascular hypertensive rats fed with high-fat-sucrose diet.
Wu XQ, Kong X, Zhou Y, Huang K, Yang JR, Li XL
European journal of pharmacology 2012 May 15;683(1-3):231-7
European journal of pharmacology 2012 May 15;683(1-3):231-7
2,3,4',5-tetrahydroxystilbene-2-O-β-D-glucoside inhibits proliferation of vascular smooth muscle cells: involvement of NO/cGMP/PKG pathway.
Xu XL, Huang YJ, Chen XF, Lin DY, Zhang W
Phytotherapy research : PTR 2012 Jul;26(7):1068-74
Phytotherapy research : PTR 2012 Jul;26(7):1068-74
Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats.
Wu H, Wang H, Zhang B, Zhang G, Zhang R, Zhang L
Neural regeneration research 2012 Feb 25;7(6):421-5
Neural regeneration research 2012 Feb 25;7(6):421-5
Effects of quercetin on gene and protein expression of NOX and NOS after myocardial ischemia and reperfusion in rabbit.
Wan LL, Xia J, Ye D, Liu J, Chen J, Wang G
Cardiovascular therapeutics 2009 Spring;27(1):28-33
Cardiovascular therapeutics 2009 Spring;27(1):28-33
Sesamin improves endothelial dysfunction in renovascular hypertensive rats fed with a high-fat, high-sucrose diet.
Kong X, Yang JR, Guo LQ, Xiong Y, Wu XQ, Huang K, Zhou Y
European journal of pharmacology 2009 Oct 12;620(1-3):84-9
European journal of pharmacology 2009 Oct 12;620(1-3):84-9
Effects of calcitonin gene-related peptide on the expression and activity of nitric oxide synthase during mandibular bone healing in rabbits: an experimental study.
Li Y, Tan Y, Zhang G, Yang B, Zhang J
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons 2009 Feb;67(2):273-9
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons 2009 Feb;67(2):273-9
Protective effects of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-d-glucoside, an active component of Polygonum multiflorum Thunb, on experimental colitis in mice.
Wang X, Zhao L, Han T, Chen S, Wang J
European journal of pharmacology 2008 Jan 14;578(2-3):339-48
European journal of pharmacology 2008 Jan 14;578(2-3):339-48
Research on the age-related changes in the nitric oxide pathway in the arteries of rats and the intervention effect of dehydroepiandrosterone.
Wu S, Ruan Y, Yin M, Lai W
Gerontology 2007;53(4):234-7
Gerontology 2007;53(4):234-7
Inhibition by brimonidine of forskolin-induced nitric oxide synthase expression in human ciliary bodies in vitro.
Wu R, Yin J, Yao K, Haefliger I
Molecular vision 2007 Apr 2;13:493-6
Molecular vision 2007 Apr 2;13:493-6
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of NOS3 using anti-NOS3 antibody (A01604-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysatesLane 2: human K562 whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human THP-1 whole cell lysatesLane 5: rat kidney tissue lysatesLane 6: mouse kidne tissue lysatesLane 7: mouse small intestine tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS3 at approximately 133KD. The expected band size for NOS3 is at 133KD.
- Additional image