AF950

antibody from R&D Systems

Targeting: NOS3 ECNOS, eNOS

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF950

Provider

R&D Systems

Product name

Human eNOS Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human eNOS in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

High glutathionylation of placental endothelial nitric oxide synthase in preeclampsia.

Guerby P, Swiader A, Augé N, Parant O, Vayssière C, Uchida K, Salvayre R, Negre-Salvayre A
Redox biology 2019 Apr;22:101126

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Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.

Liu B, Xu L, Yu X, Li W, Sun X, Xiao S, Guo M, Wang H
Molecular medicine reports 2018 Aug;18(2):1987-1994

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Memantine protects against ischemia/reperfusion-induced brain endothelial permeability.

Liu Y, Huang Y, Xu Y, Qu P, Wang M
IUBMB life 2018 Apr;70(4):336-343

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Shear stress-induced ATP-mediated endothelial constitutive nitric oxide synthase expression in human lymphatic endothelial cells.

Kawai Y, Yokoyama Y, Kaidoh M, Ohhashi T
American journal of physiology. Cell physiology 2010 Mar;298(3):C647-55

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Regional differences in prostaglandin E2 and nitric oxide production in the knee meniscus in response to dynamic compression.

Hennerbichler A, Fermor B, Hennerbichler D, Weinberg JB, Guilak F
Biochemical and biophysical research communications 2007 Jul 13;358(4):1047-53

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Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries.

Wright JL, Tai H, Churg A
American journal of respiratory cell and molecular biology 2004 Nov;31(5):501-9

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human eNOS by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothlial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eNOS Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eNOS at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human eNOS by Simple Western^TM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunoprecipitation

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Immunoprecipitation of Human eNOS. eNOS was immunoprecipitated from lysates (1 x 10⁶ cells) of HUVEC human umbilical vein endothelial cells following incubation with 0.3 µg Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). Immunoprecipitated eNOS was detected by Western blot using 1 µg/mL Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). View our recommended buffer recipes for immunoprecipitation.