Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- V3363 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Filaggrin Antibody
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- FLG01
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the Filaggrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Whole-exome sequencing in patients with ichthyosis reveals modifiers associated with increased IgE levels and allergic sensitizations.
Histamine exerts multiple effects on expression of genes associated with epidermal barrier function.
Targeting epidermal lipids for treatment of Mendelian disorders of cornification.
Induction of a chloracne phenotype in an epidermal equivalent model by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is dependent on aryl hydrocarbon receptor activation and is not reproduced by aryl hydrocarbon receptor knock down.
The regulation of epithelial cell proliferation and growth by IL-1 receptor antagonist.
Papillary fibroblasts differentiate into reticular fibroblasts after prolonged in vitro culture.
Saurian papulosis: a new clinicopathological entity.
Interleukin-22 downregulates filaggrin expression and affects expression of profilaggrin processing enzymes.
Growth and stratification of epithelial cells in minimal culture conditions.
Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.
Human eccrine sweat gland cells can reconstitute a stratified epidermis.
Generation of human epidermal constructs on a collagen layer alone.
Characterization of multipotent cells from human adult hair follicles.
A characteristic subset of psoriasis-associated genes is induced by oncostatin-M in reconstituted epidermis.
A human keratin 10 knockout causes recessive epidermolytic hyperkeratosis.
Apoptosis coordinates with proliferation and differentiation during human hair follicle morphogenesis.
Expression of epidermal N-methyl-D-aspartate receptors (NMDAR1) depends on formation of the granular layer--analysis in diseases with parakeratotic cornification.
Abnormal development of thymic dendritic and epithelial cells in human X-linked severe combined immunodeficiency.
Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.
Kiritsi D, Valari M, Fortugno P, Hausser I, Lykopoulou L, Zambruno G, Fischer J, Bruckner-Tuderman L, Jakob T, Has C
The Journal of allergy and clinical immunology 2015 Jan;135(1):280-3
The Journal of allergy and clinical immunology 2015 Jan;135(1):280-3
Histamine exerts multiple effects on expression of genes associated with epidermal barrier function.
Gutowska-Owsiak D, Salimi M, Selvakumar TA, Wang X, Taylor S, Ogg GS
Journal of investigational allergology & clinical immunology 2014;24(4):231-9
Journal of investigational allergology & clinical immunology 2014;24(4):231-9
Targeting epidermal lipids for treatment of Mendelian disorders of cornification.
Kiritsi D, Schauer F, Wölfle U, Valari M, Bruckner-Tuderman L, Has C, Happle R
Orphanet journal of rare diseases 2014 Mar 7;9:33
Orphanet journal of rare diseases 2014 Mar 7;9:33
Induction of a chloracne phenotype in an epidermal equivalent model by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is dependent on aryl hydrocarbon receptor activation and is not reproduced by aryl hydrocarbon receptor knock down.
Forrester AR, Elias MS, Woodward EL, Graham M, Williams FM, Reynolds NJ
Journal of dermatological science 2014 Jan;73(1):10-22
Journal of dermatological science 2014 Jan;73(1):10-22
The regulation of epithelial cell proliferation and growth by IL-1 receptor antagonist.
Kondo M, Yamato M, Takagi R, Namiki H, Okano T
Biomaterials 2013 Jan;34(1):121-9
Biomaterials 2013 Jan;34(1):121-9
Papillary fibroblasts differentiate into reticular fibroblasts after prolonged in vitro culture.
Janson D, Saintigny G, Mahé C, El Ghalbzouri A
Experimental dermatology 2013 Jan;22(1):48-53
Experimental dermatology 2013 Jan;22(1):48-53
Saurian papulosis: a new clinicopathological entity.
Molina-Ruiz AM, del Carmen Fariña M, Carrasco L, Santonja C, Rodríguez-Peralto JL, Torrelo A, Kutzner H, Requena L
Journal of the American Academy of Dermatology 2013 Feb;68(2):e17-22
Journal of the American Academy of Dermatology 2013 Feb;68(2):e17-22
Interleukin-22 downregulates filaggrin expression and affects expression of profilaggrin processing enzymes.
Gutowska-Owsiak D, Schaupp AL, Salimi M, Taylor S, Ogg GS
The British journal of dermatology 2011 Sep;165(3):492-8
The British journal of dermatology 2011 Sep;165(3):492-8
Growth and stratification of epithelial cells in minimal culture conditions.
Riva F, Casasco A, Casasco M, Calligaro A, Cornaglia AI
Methods in molecular biology (Clifton, N.J.) 2010;585:25-43
Methods in molecular biology (Clifton, N.J.) 2010;585:25-43
Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.
Metallo CM, Azarin SM, Moses LE, Ji L, de Pablo JJ, Palecek SP
Tissue engineering. Part A 2010 Jan;16(1):213-23
Tissue engineering. Part A 2010 Jan;16(1):213-23
Human eccrine sweat gland cells can reconstitute a stratified epidermis.
Biedermann T, Pontiggia L, Böttcher-Haberzeth S, Tharakan S, Braziulis E, Schiestl C, Meuli M, Reichmann E
The Journal of investigative dermatology 2010 Aug;130(8):1996-2009
The Journal of investigative dermatology 2010 Aug;130(8):1996-2009
Generation of human epidermal constructs on a collagen layer alone.
Riva F, Casasco A, Nespoli E, Cornaglia AI, Casasco M, Faga A, Scevola S, Mazzini G, Calligaro A
Tissue engineering 2007 Nov;13(11):2769-79
Tissue engineering 2007 Nov;13(11):2769-79
Characterization of multipotent cells from human adult hair follicles.
Raposio E, Guida C, Baldelli I, Curto M, Fiocca R, Kunkl A, Robello G, Santi PL
Toxicology in vitro : an international journal published in association with BIBRA 2007 Mar;21(2):320-3
Toxicology in vitro : an international journal published in association with BIBRA 2007 Mar;21(2):320-3
A characteristic subset of psoriasis-associated genes is induced by oncostatin-M in reconstituted epidermis.
Gazel A, Rosdy M, Bertino B, Tornier C, Sahuc F, Blumenberg M
The Journal of investigative dermatology 2006 Dec;126(12):2647-57
The Journal of investigative dermatology 2006 Dec;126(12):2647-57
A human keratin 10 knockout causes recessive epidermolytic hyperkeratosis.
Müller FB, Huber M, Kinaciyan T, Hausser I, Schaffrath C, Krieg T, Hohl D, Korge BP, Arin MJ
Human molecular genetics 2006 Apr 1;15(7):1133-41
Human molecular genetics 2006 Apr 1;15(7):1133-41
Apoptosis coordinates with proliferation and differentiation during human hair follicle morphogenesis.
Chang CH, Tsai RK, Yu HS
Journal of dermatological science 2005 Jul;39(1):9-16
Journal of dermatological science 2005 Jul;39(1):9-16
Expression of epidermal N-methyl-D-aspartate receptors (NMDAR1) depends on formation of the granular layer--analysis in diseases with parakeratotic cornification.
Fischer M, William T, Helmbold P, Wohlrab J, Marsch WCh
Archives of dermatological research 2004 Sep;296(4):157-62
Archives of dermatological research 2004 Sep;296(4):157-62
Abnormal development of thymic dendritic and epithelial cells in human X-linked severe combined immunodeficiency.
Hale LP, Buckley RH, Puck JM, Patel DD
Clinical immunology (Orlando, Fla.) 2004 Jan;110(1):63-70
Clinical immunology (Orlando, Fla.) 2004 Jan;110(1):63-70
Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.
Hale LP, Markert ML
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):617-24
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):617-24
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human skin with Filaggrin antibody (clone FLG01). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.