- MA5-13440 - Invitrogen Antibodies FLG antibody

Submitted references FGF21 promotes migration and differentiation of epidermal cells during wound healing via SIRT1-dependent autophagy.

Chen X, Tong G, Fan J, Shen Y, Wang N, Gong W, Hu Z, Zhu K, Li X, Jin L, Cong W, Xiao J, Zhu Z
British journal of pharmacology 2022 Mar;179(5):1102-1121

Fully Three-Dimensional Bioprinted Skin Equivalent Constructs with Validated Morphology and Barrier Function.

Derr K, Zou J, Luo K, Song MJ, Sittampalam GS, Zhou C, Michael S, Ferrer M, Derr P
Tissue engineering. Part C, Methods 2019 Jun;25(6):334-343

Pituitary tumor transforming gene 1 induces tumor necrosis factor-α production from keratinocytes: implication for involvement in the pathophysiology of psoriasis.

Ishitsuka Y, Kawachi Y, Maruyama H, Taguchi S, Fujisawa Y, Furuta J, Nakamura Y, Ishii Y, Otsuka F
The Journal of investigative dermatology 2013 Nov;133(11):2566-2575

Mutations in SERPINB7, encoding a member of the serine protease inhibitor superfamily, cause Nagashima-type palmoplantar keratosis.

Kubo A, Shiohama A, Sasaki T, Nakabayashi K, Kawasaki H, Atsugi T, Sato S, Shimizu A, Mikami S, Tanizaki H, Uchiyama M, Maeda T, Ito T, Sakabe J, Heike T, Okuyama T, Kosaki R, Kosaki K, Kudoh J, Hata K, Umezawa A, Tokura Y, Ishiko A, Niizeki H, Kabashima K, Mitsuhashi Y, Amagai M
American journal of human genetics 2013 Nov 7;93(5):945-56

The regulation of epithelial cell proliferation and growth by IL-1 receptor antagonist.

Kondo M, Yamato M, Takagi R, Namiki H, Okano T
Biomaterials 2013 Jan;34(1):121-9

Papillary fibroblasts differentiate into reticular fibroblasts after prolonged in vitro culture.

Janson D, Saintigny G, Mahé C, El Ghalbzouri A
Experimental dermatology 2013 Jan;22(1):48-53

Saurian papulosis: a new clinicopathological entity.

Molina-Ruiz AM, del Carmen Fariña M, Carrasco L, Santonja C, Rodríguez-Peralto JL, Torrelo A, Kutzner H, Requena L
Journal of the American Academy of Dermatology 2013 Feb;68(2):e17-22

Regulation of epithelial differentiation and proliferation by the stroma: a role for the retinoblastoma protein.

Pickard A, Cichon AC, Menges C, Patel D, McCance DJ
The Journal of investigative dermatology 2012 Dec;132(12):2691-9

A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes.

Buschke S, Stark HJ, Cerezo A, Prätzel-Wunder S, Boehnke K, Kollar J, Langbein L, Heldin CH, Boukamp P
Molecular biology of the cell 2011 Mar 15;22(6):782-94

Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.

Metallo CM, Azarin SM, Moses LE, Ji L, de Pablo JJ, Palecek SP
Tissue engineering. Part A 2010 Jan;16(1):213-23

Human eccrine sweat gland cells can reconstitute a stratified epidermis.

Biedermann T, Pontiggia L, Böttcher-Haberzeth S, Tharakan S, Braziulis E, Schiestl C, Meuli M, Reichmann E
The Journal of investigative dermatology 2010 Aug;130(8):1996-2009

Establishment and characterization of immortalized human gingival keratinocyte cell lines.

Gröger S, Michel J, Meyle J
Journal of periodontal research 2008 Dec;43(6):604-14

Characterization of multipotent cells from human adult hair follicles.

Raposio E, Guida C, Baldelli I, Curto M, Fiocca R, Kunkl A, Robello G, Santi PL
Toxicology in vitro : an international journal published in association with BIBRA 2007 Mar;21(2):320-3

A characteristic subset of psoriasis-associated genes is induced by oncostatin-M in reconstituted epidermis.

Gazel A, Rosdy M, Bertino B, Tornier C, Sahuc F, Blumenberg M
The Journal of investigative dermatology 2006 Dec;126(12):2647-57

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Filaggrin was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Filaggrin (FLG01) Mouse Monoclonal Antibody (Product # MA5-13440) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Filaggrin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Filaggrin Mouse Monoclonal Antibody (Product # MA5-13440) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human skin stained with Filaggrin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of keratinized epithelial cells of hair follicle.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Filaggrin was done on A-431 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Filaggrin Mouse Monoclonal Antibody (Product # MA5-13440, red histogram) or with mouse isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIG. 4. IHC comparison of protein expression between bioprinted tissues and native human skin show comparable morphology. Tight junction proteins ZO-1, Claudin I, and E-Cadherin. Epidermal differentiation proteins Keratin 1, Filaggrin, and Cytokeratin 15. ECM proteins Collagen I and Collagen VII and cell proliferation marker Ki67. Scale bar, 100 mum. ECM, extracellular matrix; IHC, immunohistochemistry.

MA5-13440

Antibody data