Explore
Validate
Learn
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- ELISA [1]
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1433 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Osteopontin/OPN Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Osteopontin/OPN in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant rat Osteopontin and recombinant mouse OPN is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Mineral deposition and vascular invasion of hydroxyapatite reinforced collagen scaffolds seeded with human adipose-derived stem cells.
Osteopontin mediates glioblastoma-associated macrophage infiltration and is a potential therapeutic target.
Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans.
Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis.
Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells.
Osteopontin contributes to effective neutrophil recruitment, IL-1β production and apoptosis in Aspergillus fumigatus keratitis.
Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse.
Osteopontin-metallothionein I/II interactions in experimental autoimmune encephalomyelitis.
Inhibition of Cellular Adhesion by Immunological Targeting of Osteopontin Neoepitopes Generated through Matrix Metalloproteinase and Thrombin Cleavage.
Cell stress induces upregulation of osteopontin via the ERK pathway in type II alveolar epithelial cells.
Osteopontin promotes the invasive growth of melanoma cells by activating integrin αvβ3 and down-regulating tetraspanin CD9.
CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
Mechanism of hepatitis C virus (HCV)-induced osteopontin and its role in epithelial to mesenchymal transition of hepatocytes.
Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
Osteopontin is a prognostic factor for survival of acute myeloid leukemia patients.
Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions.
The crucial role of cyclooxygenase-2 in osteopontin-induced protein kinase C alpha/c-Src/IkappaB kinase alpha/beta-dependent prostate tumor progression and angiogenesis.
Weiss-Bilka HE, Meagher MJ, Gargac JA, Niebur GL, Roeder RK, Wagner DR
Biomaterials research 2019;23:15
Biomaterials research 2019;23:15
Osteopontin mediates glioblastoma-associated macrophage infiltration and is a potential therapeutic target.
Wei J, Marisetty A, Schrand B, Gabrusiewicz K, Hashimoto Y, Ott M, Grami Z, Kong LY, Ling X, Caruso H, Zhou S, Wang YA, Fuller GN, Huse J, Gilboa E, Kang N, Huang X, Verhaak R, Li S, Heimberger AB
The Journal of clinical investigation 2019 Jan 2;129(1):137-149
The Journal of clinical investigation 2019 Jan 2;129(1):137-149
Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans.
Kapelouzou A, Kontogiannis C, Tsilimigras DI, Georgiopoulos G, Kaklamanis L, Tsourelis L, Cokkinos DV
Cytokine 2019 Apr;116:150-160
Cytokine 2019 Apr;116:150-160
Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis.
Cochain C, Vafadarnejad E, Arampatzi P, Pelisek J, Winkels H, Ley K, Wolf D, Saliba AE, Zernecke A
Circulation research 2018 Jun 8;122(12):1661-1674
Circulation research 2018 Jun 8;122(12):1661-1674
Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells.
Alshawaf AJ, Viventi S, Qiu W, D'Abaco G, Nayagam B, Erlichster M, Chana G, Everall I, Ivanusic J, Skafidas E, Dottori M
Scientific reports 2018 Jan 12;8(1):603
Scientific reports 2018 Jan 12;8(1):603
Osteopontin contributes to effective neutrophil recruitment, IL-1β production and apoptosis in Aspergillus fumigatus keratitis.
Zhao G, Hu M, Li C, Lee J, Yuan K, Zhu G, Che C
Immunology and cell biology 2018 Apr;96(4):401-412
Immunology and cell biology 2018 Apr;96(4):401-412
Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse.
Chen Y, Xu H, Lin G
Cell discovery 2017;3:17046
Cell discovery 2017;3:17046
Osteopontin-metallothionein I/II interactions in experimental autoimmune encephalomyelitis.
Jakovac H, Grubić Kezele T, Šućurović S, Mulac-Jeričević B, Radošević-Stašić B
Neuroscience 2017 May 14;350:133-145
Neuroscience 2017 May 14;350:133-145
Inhibition of Cellular Adhesion by Immunological Targeting of Osteopontin Neoepitopes Generated through Matrix Metalloproteinase and Thrombin Cleavage.
Jürets A, Le Bras M, Staffler G, Stein G, Leitner L, Neuhofer A, Tardelli M, Turkof E, Zeyda M, Stulnig TM
PloS one 2016;11(2):e0148333
PloS one 2016;11(2):e0148333
Cell stress induces upregulation of osteopontin via the ERK pathway in type II alveolar epithelial cells.
Kato A, Okura T, Hamada C, Miyoshi S, Katayama H, Higaki J, Ito R
PloS one 2014;9(6):e100106
PloS one 2014;9(6):e100106
Osteopontin promotes the invasive growth of melanoma cells by activating integrin αvβ3 and down-regulating tetraspanin CD9.
Yin M, Soikkeli J, Jahkola T, Virolainen S, Saksela O, Hölttä E
The American journal of pathology 2014 Mar;184(3):842-58
The American journal of pathology 2014 Mar;184(3):842-58
CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
Cell stem cell 2014 Mar 6;14(3):342-56
Cell stem cell 2014 Mar 6;14(3):342-56
Mechanism of hepatitis C virus (HCV)-induced osteopontin and its role in epithelial to mesenchymal transition of hepatocytes.
Iqbal J, McRae S, Banaudha K, Mai T, Waris G
The Journal of biological chemistry 2013 Dec 27;288(52):36994-7009
The Journal of biological chemistry 2013 Dec 27;288(52):36994-7009
Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
Deschaseaux F, Gaillard J, Langonné A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensébé L
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):2977-87
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):2977-87
Osteopontin is a prognostic factor for survival of acute myeloid leukemia patients.
Liersch R, Gerss J, Schliemann C, Bayer M, Schwöppe C, Biermann C, Appelmann I, Kessler T, Löwenberg B, Büchner T, Hiddemann W, Müller-Tidow C, Berdel WE, Mesters R
Blood 2012 May 31;119(22):5215-20
Blood 2012 May 31;119(22):5215-20
Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions.
Arjomandi M, Frelinger J, Donde A, Wong H, Yellamilli A, Raymond W
PloS one 2011;6(10):e25678
PloS one 2011;6(10):e25678
The crucial role of cyclooxygenase-2 in osteopontin-induced protein kinase C alpha/c-Src/IkappaB kinase alpha/beta-dependent prostate tumor progression and angiogenesis.
Jain S, Chakraborty G, Kundu GC
Cancer research 2006 Jul 1;66(13):6638-48
Cancer research 2006 Jul 1;66(13):6638-48
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Human Osteopontin/OPN ELISA Standard Curve. Human Osteopontin/OPN ELISA Standard Curve.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Osteopontin/OPN in Human Breast Cancer Tissue. Osteopontin/OPN in Human Breast Cancer Tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Osteopontin/OPN in Human Breast Cancer Tissue. Osteopontin/OPN in Human Breast Cancer Tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Human Osteopontin/OPN Antibody. Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Human Osteopontin/OPN Antibody.
