691302

antibody from BioLegend

Targeting: SPP1 BNSP, BSPI, ETA-1, OPN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

691302

Provider

BioLegend

Proper citation

BioLegend Cat#691302, RRID:AB_2629830

Product name

Purified anti-Osteopontin (SPP1)

Antibody type

Monoclonal

Description

Osteopontin, also known as secreted phosphoprotein 1 (SPP1) and early T lymphocytes activation 1 (ETA-1), is an arginine-glycine-aspartic acid (RGD) that contains multi-functional glycoproteins that are able to interact with integrins and CD44 as receptors. This protein has been shown to play roles in cell migration and survival, bone remodeling, regulation of immune cell functions, apoptosis, control of tumor cell phenotypes, and autoimmune diseases. Osteopontin is involved in recruiting and stimulating macrophages and lymphocytes in response to microbial infections. The Osteopontin receptor-ligand interaction mediates chemotaxis or attachment that depends on whether the protein is in soluble or immobilized form. Osteopontin has also been shown to play a role in the progression of cancer, which is supported by the finding that patients who suffer from disseminated carcinoma also have increased levels of Osteopontin in their plasma. In addition, Osteopontin is a component of the human atherosclerotic plaque and may be a mediator of arterial neointima formation. It is synthesized by macrophage, smooth muscle, and endothelial cells in primary and recurrent human coronary atherosclerotic plaques, which may contribute to the cellular accumulations and dystrophic calcification in the atherosclerotic plaques.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A15059B

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

SHSY-5Y cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL Osteopontin antibody (clone A15059B) at room temperature for two hours followed by Alexa Fluor® 594 conjugated (red) goat anti-mouse IgG Antibody for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Unconjugated