Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R1565 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1565, RRID:AB_1005909
- Product name
- anti Osteopontin / SPP1 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to Human Osteopontin conjugated to KLH using maleimide.
- Reactivity
- Human, Mouse, Rat, Canine, Porcine
- Host
- Rabbit
- Vial size
- 0.2 ml
- Concentration
- 82 mg/ml (by Refractometry)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoblotting: R1565 Osteopontin antibody was used at a 1/1,000 dilution to detect Osteopontin by Western blot. In lane 2 reactivity is shown against 250 ng of Human osteopontin. Lane 3 shows reactivity of MMP-cleaved osteopontin. Lane 1 shows the position of molecular weight markers. Use a 1/10,000 dilution of HRP conjugated Goat anti-Rabbit IgG (R1454HRP) for detection.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: R1565 Osteopontin antibody was used at a 1/100-1/300 dilution to detect Osteopontin which is a normal component of elastic fibers in the breast (shown heavily stained in this section of Human breast tumor cells). There is also weak staining of the extracellular matrix. Osteoponin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.