R1565

antibody from Acris Antibodies GmbH

Targeting: SPP1 BNSP, BSPI, ETA-1, OPN

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1565

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1565, RRID:AB_1005909

Product name

anti Osteopontin / SPP1 (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to Human Osteopontin conjugated to KLH using maleimide.

Reactivity

Human, Mouse, Rat, Canine, Porcine

Host

Rabbit

Vial size

0.2 ml

Concentration

82 mg/ml (by Refractometry)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunoblotting: R1565 Osteopontin antibody was used at a 1/1,000 dilution to detect Osteopontin by Western blot. In lane 2 reactivity is shown against 250 ng of Human osteopontin. Lane 3 shows reactivity of MMP-cleaved osteopontin. Lane 1 shows the position of molecular weight markers. Use a 1/10,000 dilution of HRP conjugated Goat anti-Rabbit IgG (R1454HRP) for detection.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry: R1565 Osteopontin antibody was used at a 1/100-1/300 dilution to detect Osteopontin which is a normal component of elastic fibers in the breast (shown heavily stained in this section of Human breast tumor cells). There is also weak staining of the extracellular matrix. Osteoponin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.