Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3615 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Collagen II Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Collagen II in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse.
Bone marrow-derived multipotent stromal cells attenuate inflammation in obliterative airway disease in mouse tracheal allografts.
Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjögren's-like disease.
Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription.
Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondral ossification and aggrecan metabolism.
Chen Y, Xu H, Lin G
Cell discovery 2017;3:17046
Cell discovery 2017;3:17046
Bone marrow-derived multipotent stromal cells attenuate inflammation in obliterative airway disease in mouse tracheal allografts.
Casey A, Dirks F, Liang OD, Harrach H, Schuette-Nuetgen K, Leeman K, Kim CF, Gerard C, Subramaniam M
Stem cells international 2014;2014:468927
Stem cells international 2014;2014:468927
Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjögren's-like disease.
Khalili S, Liu Y, Kornete M, Roescher N, Kodama S, Peterson A, Piccirillo CA, Tran SD
PloS one 2012;7(6):e38615
PloS one 2012;7(6):e38615
Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription.
Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A
PloS one 2012;7(4):e34703
PloS one 2012;7(4):e34703
Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondral ossification and aggrecan metabolism.
Sato T, Kudo T, Ikehara Y, Ogawa H, Hirano T, Kiyohara K, Hagiwara K, Togayachi A, Ema M, Takahashi S, Kimata K, Watanabe H, Narimatsu H
The Journal of biological chemistry 2011 Feb 18;286(7):5803-12
The Journal of biological chemistry 2011 Feb 18;286(7):5803-12
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Collagen II in Mouse Chondrocytes. Collagen II was detected in immersion fixed mouse mesenchymal stem cell-differentiated chondrocytes using 10 µg/mL Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3615) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
