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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- R1039 - Provider product page
- Provider
- OriGene
- Product name
- Collagen II (COL2A1) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Collagen II (COL2A1) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- COL2A1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
Submitted references The influence of IL-10 and TNFα on chondrogenesis of human mesenchymal stromal cells in three-dimensional cultures.
Jagielski M, Wolf J, Marzahn U, Völker A, Lemke M, Meier C, Ertel W, Godkin O, Arens S, Schulze-Tanzil G
International journal of molecular sciences 2014 Sep 9;15(9):15821-44
International journal of molecular sciences 2014 Sep 9;15(9):15821-44
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Collagen II antibody (1/400, 45 min RT) showed moderate staining of in FFPE Human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Image provided courtesy of LifeSpan Biosciences, Seattle, WA
- Validation comment
- IHC
