AM1919b

antibody from Abgent

Targeting: TSC2 LAM, PPP1R160, TSC4, tuberin

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1919b

Provider

Abgent

Proper citation

Abgent Cat#AM1919b, RRID:AB_11134310

Product name

TSC2 Antibody

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Mouse

Host

Mouse

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

TSC2 Antibody (Cat. #AM1919b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TSC2 antibody detected the TSC2 protein (arrow).

Primary Ab dilution

1:100

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent image of Hela cell stained with TSC2 Antibody(Cat#AM1919b/SG110509AA).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC2 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TSC2 immunoreactivity is localized to Microtubules significantly."

Primary Ab dilution

1:10~50