GTX81899

antibody from GeneTex

Targeting: TSC2 LAM, PPP1R160, TSC4, tuberin

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX81899

Provider

GeneTex

Proper citation

GeneTex Cat#GTX81899, RRID:AB_11167595

Product name

TSC2 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of TSC2-pS1798 (GTX81899) in Ramos cell line lysates (35μg/lane). TSC2 (arrow) was detected using the purified Pab.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fluorescent confocal image of Hela cell stained with Tuberin (TSC2) Antibody (S1798)(GTX81899).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Tuberin (TSC2) primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).Tuberin (TSC2) immunoreactivity is localized to Cytoplasm significantly.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TSC2 Antibody (S1798) (GTX81899), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.