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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-28945 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FABP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HepG2. Predicted reactivity: Mouse (84%), Rat (82%), Dog (90%), Pig (89%), Bovine (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.39 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FABP1 Polyclonal Antibody (Product # PA5-28945). Sample (30 µg of whole cell lysate). A: HepG2.15% SDS PAGE. FABP1 Polyclonal Antibody (Product # PA5-28945) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-FABP1 Polyclonal Antibody(Product # PA5-28945) and a 14.2kDa band corresponding to Fatty acid-binding protein, liver was observed in Hep G2, Mouse Liver and Rat liver, but not in the other tested cell lines and tissues. Whole cell extracts (30 µg lysate) of Hep G2 (Fig. A, Lane 1), HeLa (Fig. A, Lane 2), SH-SY5Y (Fig. A, Lane 3), HaCaT (Fig. A, Lane 4), Mouse Liver (Fig. B, Lane 1), Mouse Kidney (Fig. B, Lane 2), Mouse Adipose (Fig. B, Lane 3), Mouse Mammary gland (Fig. B, Lane 4), Rat Liver (Fig. B, Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FABP1 Polyclonal Antibody detects L-FABP protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Frozen section of rat kidney. FABP1 Polyclonal Antibody (Product # PA5-28945) diluted at 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- liver FABP antibody [N1C3] detects liver FABP protein at cytoplasm on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Liver FABP antibody [N1C3] (Product # PA5-28945) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
