AMAb90560

antibody from Atlas Antibodies

Targeting: EMD LEMD5, STA

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb90560

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb90560, RRID:AB_2665586

Product name

Anti-EMD

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGY
NDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFP
DADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSA
CQSITHY


Epitope

Binds to an epitope located within the peptide sequence DALLYQSKGYNDDYY as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL0201

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa]Lane 2: Human cell line RT-4

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of PC-3 cells using the Anti-EMD monoclonal antibody, showing specific staining in the nuclear membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows strong positivity in the nuclear membrane of glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong immunoreactivity in the nuclear membrane in renal glomeruli and tubuli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong immunoreactivity in the nuclear membrane in the trophoblast layer.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows strong positivity in the nuclear membrane of glandular cells.