Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- AF3815 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Aminopeptidase N/CD13 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Aminopeptidase N/CD13 in the direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse APN is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references TMPRSS2 activates the human coronavirus 229E for cathepsin-independent host cell entry and is expressed in viral target cells in the respiratory epithelium.
Isolation and characterization of current human coronavirus strains in primary human epithelial cell cultures reveal differences in target cell tropism.
Bertram S, Dijkman R, Habjan M, Heurich A, Gierer S, Glowacka I, Welsch K, Winkler M, Schneider H, Hofmann-Winkler H, Thiel V, Pöhlmann S
Journal of virology 2013 Jun;87(11):6150-60
Journal of virology 2013 Jun;87(11):6150-60
Isolation and characterization of current human coronavirus strains in primary human epithelial cell cultures reveal differences in target cell tropism.
Dijkman R, Jebbink MF, Koekkoek SM, Deijs M, Jónsdóttir HR, Molenkamp R, Ieven M, Goossens H, Thiel V, van der Hoek L
Journal of virology 2013 Jun;87(11):6081-90
Journal of virology 2013 Jun;87(11):6081-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Aminopeptidase N/CD13 by Western Blot. Detection of Human Aminopeptidase N/CD13 by Western Blot.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Aminopeptidase N/CD13 by Simple WesternTM. Detection of Human Aminopeptidase N/CD13 by Simple WesternTM.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Aminopeptidase N/CD13 in Human Kidney. Aminopeptidase N/CD13 in Human Kidney.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Aminopeptidase N/CD13 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Sheep Anti-Human Aminopeptidase N/CD13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3815, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Aminopeptidase N/CD13 in U937 Human Cell Line by Flow Cytometry. Detection of Aminopeptidase N/CD13 in U937 Human Cell Line by Flow Cytometry.