PA5-117783

antibody from Invitrogen Antibodies

Targeting: ANPEP CD13, gp150, LAP1, p150, PEPN

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117783

Provider

Invitrogen Antibodies

Product name

CD13 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD13 Polyclonal Antibody (Product # PA5-117783) at 1:500 dilution. Lane A: U937 Whole Cell Lysate, Lane B: THP-1 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 110 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human CD13 in human kidney with CD13 Polyclonal Antibody (Product # PA5-117783, 1:5,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD13 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 µL with CD13 Polyclonal Antibody (Product # PA5-117783) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: CD13 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 140 kDa. Observed band size: 180 kDa.