MAB6903

antibody from Abnova Corporation

Targeting: CD27 S152, TNFRSF7, Tp55

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6903

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB6903, RRID:AB_10677381

Product name

CD27 monoclonal antibody, clone M-T271

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against native CD27.

Isotype

IgG

Antibody clone number

M-T271

Storage

Store at 4°C.

References

Submitted references

Direct cellular communications between CD45R0 and CD45RA T cell subsets via CD27/CD70.

Agematsu K, Kobata T, Sugita K, Hirose T, Schlossman SF, Morimoto C
Journal of immunology (Baltimore, Md. : 1950) 1995 Apr 15;154(8):3627-35

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CD27: marker and mediator of T-cell activation?

Hintzen RQ, de Jong R, Lens SM, van Lier RA
Immunology today 1994 Jul;15(7):307-11

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Role of CD27 in T cell immune response. Analysis by recombinant soluble CD27.

Agematsu K, Kobata T, Sugita K, Freeman GJ, Beckmann MP, Schlossman SF, Morimoto C
Journal of immunology (Baltimore, Md. : 1950) 1994 Aug 15;153(4):1421-9

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

5 X 10⁵ ficoll prepared human peripheral white blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 uL of CD27 monoclonal antibody, clone M-T271 (Cat # MAB6903) at 10 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 64% sub population of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

Validation comment

Flow Cytometry