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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- ARG20567 - Provider product page
- Provider
- Arigo
- Product name
- anti-VE-Cadherin phospho (Tyr685) antibody
- Antibody type
- Polyclonal
- Antigen
- Phosphospecific peptide (coupled to carrier protein) around Tyr685 of Human VE-cadherin.
- Description
- This antibody was pre-adsorbed with an unrelated phospho-tyrosine peptide and unphosphorylated VE-cadherin (Tyr685) peptide then affinity purified with immunogen peptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
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- Arigo (provider)
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- Experimental details
- Western blot: Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (Lanes 1 & 3) or treated with alkaline phosphatase (Lanes 2 & 4). The blots were stained with ARG20567 anti-VE-Cadherin phospho (Tyr685) antibody (Lanes 1 & 2) or mouse monoclonal anti-VE-cadherin antibody (Lanes 3 & 4).
