AM26286FC-N

antibody from Acris Antibodies GmbH

Targeting: CDH5 7B4, CD144

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Functional assay (Recommended by provider)

Antibody Data

Product number

AM26286FC-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM26286FC-N, RRID:AB_11214796

Product name

anti CD144 / Cadherin-5 (extracell. dom.)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

BV9

Vial size

0.1 mg

Concentration

0.1 mg/ml

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4°C.(Black- no stain, Red- isotype control, Blue- AM26286PU-N–BV9)