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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-64848 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64848, RRID:AB_2228212
- Product name
- Mouse Monoclonal EMMPRIN/CD147 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-64848 recognizes a membrane glycoprotein of MW 42-48 kD expressed at high levels on activated lymphocytes but lower levels on resting cells. The antigen is also present on some endothelia. The antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue. It is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Taurine Reduces tPA (Tissue-Type Plasminogen Activator)-Induced Hemorrhage and Microvascular Thrombosis After Embolic Stroke in Rat.
Ventilator-induced lung injury upregulates and activates gelatinases and EMMPRIN: attenuation by the synthetic matrix metalloproteinase inhibitor, Prinomastat (AG3340).
Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.
Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.
Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels.
The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence.
Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
Jin R, Xiao AY, Liu S, Wang M, Li G
Stroke 2018 Jul;49(7):1708-1718
Stroke 2018 Jul;49(7):1708-1718
Ventilator-induced lung injury upregulates and activates gelatinases and EMMPRIN: attenuation by the synthetic matrix metalloproteinase inhibitor, Prinomastat (AG3340).
Foda HD, Rollo EE, Drews M, Conner C, Appelt K, Shalinsky DR, Zucker S
American journal of respiratory cell and molecular biology 2001 Dec;25(6):717-24
American journal of respiratory cell and molecular biology 2001 Dec;25(6):717-24
Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.
Crook K, Hunt SV
Developmental immunology 1996;4(4):235-46
Developmental immunology 1996;4(4):235-46
Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82.
Crook K, Hunt SV
Developmental immunology 1996;4(4):235-46
Developmental immunology 1996;4(4):235-46
Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels.
Cassella JP, Lawrenson JG, Allt G, Firth JA
Journal of anatomy 1996 Oct;189 ( Pt 2):407-15
Journal of anatomy 1996 Oct;189 ( Pt 2):407-15
The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence.
Fossum S, Mallett S, Barclay AN
European journal of immunology 1991 Mar;21(3):671-9
European journal of immunology 1991 Mar;21(3):671-9
Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
Paterson DJ, Jefferies WA, Green JR, Brandon MR, Corthesy P, Puklavec M, Williams AF
Molecular immunology 1987 Dec;24(12):1281-90
Molecular immunology 1987 Dec;24(12):1281-90
Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
Paterson DJ, Jefferies WA, Green JR, Brandon MR, Corthesy P, Puklavec M, Williams AF
Molecular immunology 1987 Dec;24(12):1281-90
Molecular immunology 1987 Dec;24(12):1281-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Analysis of FFPE tissue section of rat kidney using 1:100 dilution of CD147 antibody (clone OX-47). The staining was detected using HRP-labelled secondary antibody and DAB reagent which followed counterstaining of sections with hematoxylin. This antibody generated primarily a membrane staining with some cytoplasmic signal in the cuboidal epithelial cells of various tubules.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Rat Splenocytes were stained with EMMPRIN/CD147 (OX-47) antibody NB100-64848 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.
