Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-1472-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD147 Monoclonal Antibody (8D12), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 8D12 recognizes CD147 which is a member of the immunoglobulin superfamily. It is also called EMMPRIN and basigin. CD147 has been characterized as a tumor cell collagenase stimulating molecule and has also been shown to have a role in cell adhesion. CD147 can be found in association with integrins alpha3 beta1 and alpha6 beta1 by co-immpunopreciptations. Additionally it is thought to play a role in T cell activation and thymocyte cycling. CD147 is expressed broadly on hematopoietic and non-hematopoietic cells, especially in tumor cells. Applications Reported: This 8D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 8D12 antibody has been pre-titrated and tested by flow cytometric analysis or normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8D12
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.
Involvement of CD147 in overexpression of MMP-2 and MMP-9 and enhancement of invasive potential of PMA-differentiated THP-1.
CD147 monoclonal antibodies induce homotypic cell aggregation of monocytic cell line U937 via LFA-1/ICAM-1 pathway.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.
Byrne SM, Ortiz L, Mali P, Aach J, Church GM
Nucleic acids research 2015 Feb 18;43(3):e21
Nucleic acids research 2015 Feb 18;43(3):e21
Involvement of CD147 in overexpression of MMP-2 and MMP-9 and enhancement of invasive potential of PMA-differentiated THP-1.
Zhou J, Zhu P, Jiang JL, Zhang Q, Wu ZB, Yao XY, Tang H, Lu N, Yang Y, Chen ZN
BMC cell biology 2005 May 17;6(1):25
BMC cell biology 2005 May 17;6(1):25
CD147 monoclonal antibodies induce homotypic cell aggregation of monocytic cell line U937 via LFA-1/ICAM-1 pathway.
Kasinrerk W, Tokrasinwit N, Phunpae P
Immunology 1999 Feb;96(2):184-92
Immunology 1999 Feb;96(2):184-92
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD147 APC (purple histogram). Cells in the lymphocyte gate were used for analysis.